Multilayer hollow Nano fiber in use for cell culture, and preparation method

A technology for cell culture and nanofibers, applied in tissue cell/virus culture devices, biochemical equipment and methods, chemical instruments and methods, etc., can solve problems such as multi-layer hollow nanofibers that have not been reported, and achieve free choice of components, The effect of simple preparation device, easy storage and transportation
CN1958134AInactive Publication Date: 2007-05-09SOUTHEAST UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SOUTHEAST UNIV
Publication Date
2007-05-09
Estimated Expiration
Not applicable · inactive patent

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Abstract

A multi-layer hollow nanofiber used to culture cells is prepared through providing electrically spun fibers as template, alternatively adsorbing the polymers with positive and negative charges onto the surface of template, and using organic solvent to remove said template.
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Description

technical field

[0001] The invention relates to a method for preparing a multilayer hollow nanofiber that can be used for cell culture. The hollow multilayer nanofiber can be used as a support material for cell culture and belongs to the technical field of cell culture carrier materials. Background technique

[0002] The hollow nanofiber bioreactor can be used for both suspension cell culture and adherent cell culture. The principle is: to simulate the three-dimensional state of cell growth in the body, and use the longitudinal arrangement of thousands of hollow fibers in the reactor to provide an in vitro growth microenvironment that approximates physiological conditions for cells, so that cells can continue to grow. Hollow nanofiber is a fine tubular structure, the tube wall is an extremely thin semi-permeable membrane, rich in capillaries, the fiber tube is perfused with oxygen-filled serum-free culture medium during culture, and the outer wall of the tube is for cell adh...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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