Gene chip for detecting virus infected by oxen, kit, and gene chip preparation method

Abstract

This invention relates to a gene chip for detecting viruses of bovine infectious diseases. The gene chip comprises a solid carrier and an oligonucleotide probe immobilized on the solid carrier. The oligonucleotide probe is DNA fragment selected from the nucleotide sequences of bovine rabies virus, bovine viral diarrhea virus, bovine foot and mouth disease virus, rinderpest virus and bovine bluetongue virus. This invention also relates to a method for preparing the gene chip and utilizing the gene chip for detecting the five viruses. The method comprises: synthesizing the probe, preparing the gene chip, and hybridizing with a treated sample. The gene chip, together with sample treatment solution, hybridization reagent, color-development reagent and specification, constitutes the test kit for detecting viruses of bovine infectious diseases. The gene chip can detect the five viruses simultaneously, and has such advantages as easy operation, high accuracy and good repeatability.

Description

technical field The invention relates to the technical field of bioengineering, in particular to a gene chip for detecting bovine infectious disease viruses. A chip preparation method, a detection method and a detection kit. Background technique Rabies is caused by rabies virus, also known as hydrophobia, characterized by high central nervous system excitement and severe disturbance of consciousness, paroxysmal excitement and shock movements, and finally death due to respiratory nerve paralysis. The case fatality rate is extremely high, and humans and various livestock and poultry are susceptible. In my country, the incidence rate continues to rise. At present, the key epidemic areas in the world are still in Asia, mainly in Southeast Asian countries. In recent years, the world's epidemic trend has also increased. Bovine viral diarrhea is an infectious disease of cattle and sheep caused by mucosal disease viruses. It is characterized by erosion and necrosis of the gastro...

AI Technical Summary

Problems solved by technology

In general, because the chip technology immobilizes a large number of probes on the support at the same time, it can detect and analyze a large number of sequences in the sample at one time, thus solving the problem of complicated operation, low degree of automation and sequence of operations in traditional nucleic acid blot hybridization technology. The number is small, the detection efficiency is low, etc.

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