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In-situ preparing method for micro porous plate pH sensor

An in-situ preparation and pH sensor technology, applied in interdisciplinary fields, can solve the problems of large environmental pollution and high cost

Inactive Publication Date: 2011-08-31
常熟紫金知识产权服务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Plasma treatment, such as ion bombardment and implantation, electron beam radiation and other methods require specific equipment and high cost; photochemical oxidation and chemical oxidation need to use strong acid and strong oxidant, ultraviolet radiation, etc., which cause great environmental pollution

Method used

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  • In-situ preparing method for micro porous plate pH sensor
  • In-situ preparing method for micro porous plate pH sensor
  • In-situ preparing method for micro porous plate pH sensor

Examples

Experimental program
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Embodiment Construction

[0032] An in-situ preparation method of a microporous plate pH sensor, the steps of which are:

[0033] (1) Drop 10 μL of triethoxy-3-isocyanatopropylsilane (TESPI) into the ethanol solution of pH-sensitive reagent 5-aminofluorescein (FITC), and control the molar ratio of TESPI to FITC at 1: 14, the temperature is at 5 or 10 or 15 or 20 or 25 or 28 or 31 or 33 or 36 or 41 or 46 or 50 ° C, reacting for 1 or 3 or 5 or 10 or 15 or 20 or 24 or 28 or 30 hours to obtain Reaction solution A;

[0034] (2) Mix 0.6 mL of tetraethoxysilane, 40 μL of 0.01M hydrochloric acid and 0.12 mL of water evenly, and sonicate for 1 or 1.5 or 2 hours to obtain reaction solution B;

[0035] (3) Take 0.2mL of reaction solution A, 0.2mL of reaction solution B and 0.1M calcium chloride (CaCl 2 ) solution 1.0mL was mixed evenly to obtain solution C;

[0036] (4) Take solution C1.0 or 5 or 10 or 15 or 20 or 30 or 40 or 50 μL and spread evenly on the bottom of 96 or 384 or 1856 well plate, seal the micro...

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Abstract

The method includes steps: (1) dropping triethoxy - 3 - propyl silicane isocyanic acid to ethanol solution of pH sensitive reagent to obtain reaction fluid A; (2) mixing siloxane, acid, and water to obtain reaction fluid B; (3) mixing reaction fluid A, reaction fluid B and solution of calcium chloride according to proportion to obtain solution C; (4) coating solution C on micropore base of micropore plate, using sealing tape to seal micropores, and using needle to perforate each micropore at center, and protecting from light; after placing it a period of time, the method adds distilled water to micropores, keeping the back up. Features are: simple and convenient, low cost, and high generality. The sensor can be in use for monitoring each hydrolytic reaction, and chemical reaction related to pH change. Satisfactory result is obtained when the sensor is in use for filtering inhibitor of beta -lactamase, and antibiotics of beta -lactam.

Description

technical field [0001] The invention relates to the interdisciplinary fields of biology, medicine, pharmacy, material, chemistry and other subjects. More specifically, it relates to a preparation method of a pH sensor at the bottom of a micropore plate. Background technique [0002] β-lactam antibiotics, such as penicillin and streptomycin, are the most commonly used antibiotics. Due to the widespread use and even abuse in clinical treatment and animal breeding, the problem of drug resistance has become increasingly prominent, and even poses a serious threat to the continued use of antibiotics for treatment. The reason for drug resistance is due to the production of β-lactamases by bacteria. With the continuous use of antibiotics, new enzymes and mutant enzymes are constantly produced, and the problem of drug resistance is becoming more and more serious. [0003] β-lactamase (E.C.3.5.2.6) is a kind of β-lactam hydrolase, which catalyzes the hydrolysis of β-lactam ring, br...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/545
Inventor 何治柯刘红兵梁润松黄国贤
Owner 常熟紫金知识产权服务有限公司