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Process for biological treatment of thick-oil waste water

A heavy oil wastewater and biological treatment technology, applied in the field of microbial treatment of water pollution, can solve the problems of failing to meet design standards and current needs, COD removal rate less than 30%, low biological treatment efficiency, etc., to achieve less variation and lower cost Low, strong removal effect

Inactive Publication Date: 2007-07-04
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Organic polymers and petroleum hydrocarbons are the main pollutants in heavy oil wastewater. These pollutants have large molecular weight, poor biodegradability, and are difficult to biodegrade. They often enter the environment with the discharge of oilfield produced water, causing serious pollution to the environment.
The biological treatment efficiency of these pollutants is very low. In most cases, the COD removal rate is less than 30%, which is far from meeting the design standards and current needs.

Method used

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  • Process for biological treatment of thick-oil waste water
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Alternaria alternate is used to prepare bacteria after activation and multiplication culture, and then added to heavy oil wastewater to treat heavy oil wastewater; the details are as follows:

[0023] (1) Activation of bacteria

[0024] In a test tube containing a slant medium, use an inoculation loop to pick the bacteria to inoculate Alternaria alternate, and place it in a 30°C incubator for 3 days until black spores appear. The composition of the slant medium is: 2% glucose, 20% potato, 0.05% KH 2 PO 4 , 0.025% MgSO 4 , 2% agar, natural pH, the balance is water as a solvent; sterilize at 121°C, 0.1MPa for 30min.

[0025] (2) Proliferation culture

[0026] Use an inoculation loop to pick well-growing bacteria from the slope and inoculate them into a conical flask filled with liquid medium, and culture them on a shaker with a temperature set at 28°C and a rotation speed of 180rpm for 3 days until there are a large number of bacteria in the liquid medium. Propagatio...

Embodiment 2

[0033] The difference from Example 1 is:

[0034] (1) Activation of bacteria

[0035] In a test tube containing a slant medium, use an inoculation loop to pick the thalli to inoculate Alternaria alternate, and place it in an incubator at 28°C for 4 days until black spores appear. The composition of the slant medium is: 3% glucose, 25% potato, 0.15% KH 2 PO 4 , 0.035% MgSO 4 , 1.5% agar, natural pH, the balance is water as a solvent; sterilize at 121°C, 0.1MPa for 30min.

[0036] (2) Proliferation culture

[0037] Use an inoculation loop to pick well-growing bacteria from the slope and inoculate them into a conical flask filled with liquid medium, and culture them on a shaking table with a temperature set at 30°C and a rotation speed of 120rpm for 2 days until there are a large number of bacteria in the liquid medium. Propagation; liquid medium composition: 3% glucose, 25% potato, 0.15% KH 2 PO 4 , 0.035% MgSO4 , the pH is natural, and the balance is water as a solvent; ...

Embodiment 3

[0043] The difference from Example 1 is:

[0044] (1) Activation of bacteria

[0045] In a test tube containing a slant medium, an inoculation loop was used to pick the thalline to inoculate Alternaria alternate, and place it in an incubator at 25°C for 3 days until black spores appeared. The composition of the slant medium is: 1.5% glucose, 15% potato, 0.10% KH 2 PO 4 , 0.03% MgSO 4 , 1.4% agar, natural pH, the balance is water as a solvent; sterilize at 121°C, 0.1MPa for 30min.

[0046] (2) Proliferation culture

[0047] Use the inoculation loop to pick the well-growing bacteria from the slope and inoculate them into the Erlenmeyer flask containing the liquid medium, and cultivate them on the shaker with the temperature set at 25°C and the rotation speed of 150rpm for 3 days until the bacteria in the liquid medium Massive propagation; the composition of the liquid medium is: 1.5% glucose, 15% potato, 0.10% KH 2 PO 4 , 0.03% MgSO 4 , the pH is natural, and the balance...

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Abstract

The invention relates to a biological treatment for water pollution, which in detail is a biological method for treating heavy oil wastewater. It prepares bacteria with alternaria alternate through activation and multiplying culture, then throws it into heavy oil wastewater, treats wastewater with temperature being 20-35 Deg. C, and rotation speed of table concentrator being 100-200 rmp for 6-96 hours. The inoculation amount of bacteria, which accounts as mass percentage is 1%-3%. The invention employs alternaria alternate that shows self flocculation to treat heavy oil wastewater biologically and can reduce COD from said wastewater effectively in a short time.

Description

technical field [0001] The invention relates to a microbial treatment technology for water body pollution, in particular to a method for treating heavy oil wastewater by utilizing the self-flocculation ability of fungi. Background technique [0002] At present, most domestic oil fields are exploited by water injection. With the increase of mining time, the water content of crude oil increases year by year, and can reach more than 90% in the later stage, so a large amount of oily wastewater is generated. The treatment methods of heavy oil wastewater mainly include physical methods, chemical methods and biological methods. The first two methods are mainly for the treatment of high-concentration oily wastewater. For wastewater with low oil content, the treatment cost is high, while the cost of biological treatment is low, there is no secondary pollution, and the pollutants can be completely degraded, so they are widely used in the subsequent treatment of oily wastewater. deal ...

Claims

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Application Information

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IPC IPC(8): C02F3/34C02F103/10
Inventor 郭书海王鑫李凤梅
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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