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Dried rehmannia root oligosaccharide and its preparation method and uses

An oligosaccharide, raw land technology, applied in the preparation of oligosaccharides and sugar derivatives, chemical instruments and methods, etc., to achieve the effect of inhibiting lung inflammation

Active Publication Date: 2007-07-04
SHUGUANG HOSPITAL AFFILIATED WITH SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no research reports on the extraction and separation of chemical components in habitats that have this active effect at home and abroad.

Method used

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  • Dried rehmannia root oligosaccharide and its preparation method and uses
  • Dried rehmannia root oligosaccharide and its preparation method and uses
  • Dried rehmannia root oligosaccharide and its preparation method and uses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Wash and dry 500g of crude drug raw land, cut into slices, decoct 3 times with 12 times deionized water, 0.5 hours each time, filter, combine the filtrates, centrifuge, and pass the supernatant through a D101 macroporous adsorption resin column, and use 5 times the volume of the resin bed eluted with water, collected the effluent and water eluate, decolorized twice with 0.1% activated carbon, filtered, and combined the filtrates to obtain the total sugar solution of raw land, which passed through a nanofiltration membrane with a molecular weight cut-off of 1000 and a nanofiltration membrane with a molecular weight cut-off of 200 Membranes, the concentrated solution between the two membranes was collected, and freeze-dried to obtain 87 g of geooligosaccharide fractions.

Embodiment 2

[0034] Wash and dry 500g of crude drug raw land, cut into slices, decoct 3 times with 14 times deionized water, 0.5 hours each time, filter, combine the filtrates, centrifuge, and the supernatant passes through HPD100 macroporous adsorption resin column, with 5 times the volume of the resin bed eluted with water, collected the effluent and water eluent, decolorized 3 times with 0.1% activated carbon, filtered, combined the filtrates, concentrated under reduced pressure at 60 ° C, and freeze-dried to obtain 210 g of total sugar of raw land. Weigh 5 g of raw ground total sugar, dissolve it in 20 mL of distilled water, and filter. Put the filtrate on a Sephadex G-15 gel column, elute with water, the flow rate is 2mL / min, collect 8-12mL in each tube, use thin-layer chromatography to detect, collect and combine the parts containing trisaccharide and tetrasaccharide spots, and reduce at 60°C. Concentrate under reduced pressure and freeze-dry to obtain 2.3 g of the geooligosaccharide...

Embodiment 3

[0036] Wash and dry 500g of crude drug raw land, crush it into 5mm size blocks, decoct it with 12 times deionized water for 2 times, each time for 1 hour, filter, combine the filtrates, centrifuge, and the supernatant passes through the HPD100 macroporous adsorption resin column, and use The water was eluted with 5 times the volume of the resin bed, the effluent and the water eluate were collected, decolorized 3 times with 0.15% activated carbon, filtered, the combined filtrates were concentrated under reduced pressure at 65°C, and freeze-dried to obtain 225 g of total sugar in raw ground. Weigh 6 g of total sugar of raw land, dissolve in 25 mL of distilled water, and filter. Take the filtrate and put it on Sephadex G-25 gel column, elute with water, the flow rate is 2mL / min, collect 8-12mL in each tube, use thin-layer chromatography to detect, collect and combine the parts containing tri- and tetrasaccharide spots, and reduce at 65°C. Concentrate under reduced pressure and fr...

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PUM

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Abstract

The invention relates to rehmanniae radix oligosaccharides, the method for preparing the same, and its application. The method comprises taking rehmanniae radix medicinal materials as raw material, extracting with and getting rehmanniae radix total sugar, separating with silica gel column and getting the oligosaccharides, mainly comprising stachyose, and. The content is more than 60%. It is demonstrated by pharmacodynamics experiment that the oligosaccharides can inhibit rat lung inflammation induced by lipopolysaccharide of coli bacillus, and can be used to prepare drug treating chronic obstructive pulmonary disease.

Description

technical field [0001] The invention relates to the technical field of effective fractions of traditional Chinese medicines and their extraction and separation, in particular to a regiooligosaccharide and its preparation method and application. Background technique [0002] Shengdi is a commonly used traditional Chinese medicine, with mild nature and flavor, and the function of clearing heat and cooling blood, nourishing yin and promoting body fluid. The chemical composition of Rehmannia glutinosa is complex, and the main components are iridoid glycosides and sugars [Edited by Yin Jian. Modern Research and Application of Traditional Chinese Medicine (Volume II) [M]. Xueyuan Press, 1752 (1997)]. Literature reports, [Bi Xiaoli, Zhang Wei, Liaoning Journal of Traditional Chinese Medicine, 26(3), 110(1999)], [Bi Xiaoli, Zhang Wei, Liaoning Journal of Traditional Chinese Medicine, 25(12), 561(1998)], [Zhang Wei, Bi Xiaoli , Journal of Practical Chinese Medicine, 16(3), 3(2003)],...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H3/06C07H1/08A61K31/702A61P11/00
Inventor 刘力徐德生唐岚
Owner SHUGUANG HOSPITAL AFFILIATED WITH SHANGHAI UNIV OF T C M
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