Method for preparing chloromycetin artificial antigen

A technology of artificial antigen and chloramphenicol, which is applied in the field of preparation of chloramphenicol artificial antigen, can solve the problems of difficult antibiotic residue, high price, cumbersome operation, etc., and achieve the effect of low cost, simple operation and rapid detection

Inactive Publication Date: 2007-07-11
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Whether the residual amount of chloramphenicol in meat products and aquatic products exceeds the standard usually adopts chromatographic detection method, but the operation is cumbersome and requires a special person to measure, which brings difficulties for the production enterprise to understand the residual amount of antibiotics; later, people carried out radioimmunoassay and Enzyme-linked immunoassay, but the equipment must be imported from abroad, and the price is expensive; since chloramphenicol is a hapten, it needs to be cross-linked with the carrier protein to prepare the corresponding anti-chloramphenicol antibody, and on this basis can the relevant immune detection be established method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] A preparation method of chloramphenicol artificial antigen is characterized in that the following steps are adopted:

[0013] A) Weigh chloramphenicol and succinic anhydride at a weight ratio of 1:0.3 and add them to anhydrous pyridine successively. Medium water bath at 60°C for 24h (a large amount of preparation requires a water bath at 60°C for 24h in a reflux device) to generate carboxyl-containing chloramphenicol derivatives;

[0014] B) Place the above-mentioned chloramphenicol derivatives in a centrifuge tube, add 5 times the amount of ice-cold 5N hydrochloric acid of the original reaction volume of chloramphenicol, and rotate the centrifuge tube at a speed of 1000 rpm for 10 minutes to separate out carboxyl-containing chloramphenicol For the red product, centrifuge and wash the precipitate twice with 2 times the volume of 5N hydrochloric acid ice-cold hydrochloric acid solution to obtain a purple-red precipitate;

[0015] C) adding concentration to the precipita...

Embodiment 2

[0018] A preparation method of chloramphenicol artificial antigen is characterized in that the following steps are adopted:

[0019] A) Weigh chloramphenicol and succinic anhydride respectively in a weight ratio of 1:0.5, and add them to anhydrous pyridine successively. Medium water bath at 60°C for 6h (a large amount of preparation requires a water bath at 60°C for 6h in a reflux device) to generate carboxyl-containing chloramphenicol derivatives;

[0020] B) Place the above-mentioned chloramphenicol derivatives in a centrifuge tube, add 10 times the amount of ice-cold 1N hydrochloric acid of the original reaction volume of chloramphenicol, and rotate the centrifuge tube at a speed of 1000 rpm for 10 minutes to separate out carboxyl-containing chloramphenicol For the red product, centrifuge and wash the precipitate 3 times with 10 times the volume of 1N hydrochloric acid ice-cold hydrochloric acid solution to obtain a purple-red precipitate;

[0021] C) adding a concentratio...

Embodiment 3

[0024] A preparation method of chloramphenicol artificial antigen is characterized in that the following steps are adopted:

[0025] A) Weigh chloramphenicol and succinic anhydride at a weight ratio of 1:0.4 and add them to anhydrous pyridine successively. Medium water bath at 60°C for 15h (a large amount of preparation requires a water bath at 60°C for 15h in a reflux device) to generate carboxyl-containing chloramphenicol derivatives;

[0026] B) Place the above-mentioned chloramphenicol derivatives in a centrifuge tube, add 7 times the amount of ice-cold 3N hydrochloric acid of the original reaction volume of chloramphenicol, and rotate the centrifuge tube at a speed of 1000 rpm for 10 minutes to separate out carboxyl-containing chloramphenicol For the red product, centrifuge and wash the precipitate 3 times with 7 times the volume of 3N hydrochloric acid ice-cold hydrochloric acid solution to obtain a purple-red precipitate;

[0027] C) adding a concentration of 55% ethan...

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Abstract

The alficetin artificial antigen is made by solving the alficetin into pyridomycin with succinic anhydride to have carboxy group in the alficetin molecule, adding EDAC and acepramin, making the carboxy group of the alficetin derivative condensing with the amidol of the acepramin, extending the carbon chain, getting the alficetin-carbon chain-carrier protein compound through carrier protein coupling. It is easy in application, and can be used for immune inspecting of the alficetin residue of zoonite with improved inspection level.

Description

technical field [0001] The invention relates to a compound containing chloramphenicol, in particular to a preparation method of an artificial antigen of chloramphenicol. Background technique [0002] Chloramphenicol is an important antibiotic for human and animal disease prevention and treatment. It is widely used in animal treatment. Due to factors such as low price, this antibiotic is widely used in animal feed and is one of the commonly used animal feed additives. . However, the irrational abuse of antibiotics has led to many bacteria being able to tolerate antibiotics and develop drug resistance, which has a negative impact on the effective treatment of diseases with antibiotics. With the improvement of people's living standards and the prevention awareness of the abuse of antibiotics, they gradually realized the dangers of the abuse of antibiotics, and began to pay attention to the side effects of chloramphenicol, especially the residual amount of antibiotics in meat p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53A61K39/00
Inventor 陈正贤邬建敏叶兴乾沈立荣吴建祥马志超徐正
Owner ZHEJIANG UNIV
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