Prokineticin polypeptides, related compositions and methods

a technology of prokineticin and polypeptide, applied in the field of prokineticin polypeptide, related compositions and methods, can solve the problems of reducing the efficacy of these regulators as therapeutics, limiting the utility of these regulators for therapeutic use, etc., and achieve the effect of inhibiting promoting the production of prokineticin receptor signals

Inactive Publication Date: 2005-02-17
RGT UNIV OF CALIFORNIA
View PDF0 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0011] The invention also provides methods of identifying a prokineticin receptor ligand, by contacting a preparation containing prokineticin receptor with one or more candidate compounds, and identifying a compound that specifically binds to the receptor. Such a compound is characterized as a prokineticin receptor ligand.
[0012] Also provided are methods of identifying a prokineticin receptor agonist, by contacting a preparation containing a prokineticin receptor with one or more candidate compounds, and identifying a compound

Problems solved by technology

Methods of recombinantly preparing these snake and frog polypeptides, or of recombinantly preparing other polypeptides containing 10 cysteines, have not previously been described, limiting the ut

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Prokineticin polypeptides, related compositions and methods
  • Prokineticin polypeptides, related compositions and methods
  • Prokineticin polypeptides, related compositions and methods

Examples

Experimental program
Comparison scheme
Effect test

example i

Identification, Preparation and Characterization of Prokineticins 1 and 2

[0132] This example shows the cloning, recombinant expression, purification and biological activities of human prokineticins 1 and 2, as well as modifications thereof.

[0133] Materials and Methods

[0134] RNA Blot

[0135] Human multiple tissue RNA blots containing normalized samples of poly a RNA were used as described by the manufacture's instructions (Clontech). The blots were probed with random primer-labeled probes(nucleotides 1-550 and 1-1178 for prokineticin 1 and prokineticin 2 cDNAs), and signals were visualized by exposing to Kodak XAR film.

[0136] Production, Refolding and Purification of Recombinant Prokineticins

[0137] The coding sequences for mature prokineticins were cloned into prokacyotic expression vector pGEX-3X (Pharmacia). The extra nucleotidcs between the factor Xa protease digestion site of GST (Glutathione-s-transferase) tag and mature prokineticins were removed by site-directed mutagenesi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Fractionaaaaaaaaaa
Fractionaaaaaaaaaa
Fractionaaaaaaaaaa
Login to view more

Abstract

The invention provides isolated polypeptides that stimulate gastrointestinal smooth muscle contraction, including human prokineticin 1 and human prokineticin 2 polypeptides, and functional fragments and modifications thereof. Also provided are methods of stimulating gastrointestinal smooth muscle contraction in a mammal, by administering to the mammal an effective amount of a prokineticin polypeptide. The invention also provides nucleic acid molecules encoding a prokineticin polypeptide, and antibodies that selectively bind a prokineticin polypeptide. Further provided are methods of identifying a prokineticin receptor ligand, agonist or antagonist.

Description

[0001] This application claims the benefit of U.S. Provisional Application No. 60 / 245,882, filed Nov. 3, 2000, which is incorporated herein by reference.BACKGROUND OF THE INVENTION [0002] The main function of gastrointestinal (GI) smooth muscle is to mix and propel intralumenal contents, which enables efficient digestion of food, progressive absorption of nutrients, and eventual evacuation of residual components. The activity of GI smooth muscle is regulated by intrinsic and extrinsic neural signals, including classical neurotransmitters, co-existing neuropeptides, and circulating peptide hormones. In addition, a number of humoral agents including histamine, serotonin, and adenosine that are produced by nonneural GI cells also influence the activity of smooth muscle cells. [0003] A number of clinical conditions are associated with altered GI motility, including irritable bowel syndrome, diabetic gastroparesis, postoperational ileus, chronic constipation, gastrointestinal reflux dise...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K38/00A61K39/00G01N33/50A61P1/00A61P29/00A61P31/00A61P35/00C07K14/47C07K16/18C12N1/15C12N1/19C12N1/21C12N5/10C12N15/09C12N15/12C12P21/02C12Q1/02G01N33/15G01N33/53G01N33/566
CPCA61K38/00C07K14/47A61K39/00A61P1/00A61P29/00A61P31/00A61P35/00
Inventor ZHOU, QUN-YONGEHLERT, FREDERICK
Owner RGT UNIV OF CALIFORNIA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap