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Synergistic bioinoculant composition comprising bacterial strains of accession nos. NRRL B-30486, NRRL B-30487, and NRRL-B 30488 and a method of producing said composition thereof

a bioinoculant and composition technology, applied in the field of synergistic bioinoculant composition, can solve the problems of inability to select such bacterial strains, inconvenient field testing, and inability to commercialize bioinoculants, and achieve the effect of less seeding mortality and better seedling germination

Inactive Publication Date: 2006-05-04
NAUTIYAL CHANDRA SHEKHAR +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0022] Still another object of the present invention is to isolate bacterial stains from the cow Sahiwal showing plant growth promoting activity in terms of less seeding mortality, better seedling germination, plant height, number of pods and seed dry weight.
[0030] Still another object of the present invention is to develop a formulation comprising three strains isolated from the cow Sahiwal, useful as a bioinoculant, showing maximum viability under varied storage or greenhouse or field conditions.

Problems solved by technology

However, a major factor in the unsuccessful commercialization of bioinoculants has been the inconsistency of field test results as their establishment and performance are severely effected by environmental factors especially under stress conditions encountered in soil e.g., salt, pH, and temperature [C. S. Nautiyal et al., FEMS Microbiology Letters, Volume 182, pp.
Additionally, no procedure for the selection of such bacterial strain has been reported.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

Isolation of Bacterial Strains from Milk

[0153] Fifty bacterial representatives of the predominant morphologically distinct colonies present on the plates were selected from three individual milk samples from each of healthy human, indigenous (Sahiwal) cow, exotic (Holstein Frisian) cow and buffalo. Therefore, a total of 600 bacterial strains were collected for further screening. Human milk was collected from three mothers with breast-fed infants in the range of 6 to 12 weeks old. Milk from pure breed native Sahiwal cows #12, #217, and #249 was collected from Gajaria farm, Department of Animal Husbandry, Government of Uttar Pradesh, Lucknow. Milk from exotic breed Holstein Frisian (15 / 16) cows #154, #412, and #667 was collected from Indian Military Farm, Central Command Headquarters, Indian Army, Lucknow. Milk samples from buffalo were collected from local commercial dairy farm. Milk was collected in sterile containers after taking due care to sanitize the teat and human handler. S...

example 2

Screening of Bacterial Strains Under In Vitro Conditions for Ability to Suppress Pathogenic Fungi and Bacteria

[0156] The 600 bacterial strains obtained by the procedure outlined in Example 1 were screened for their ability to inhibit growth of Colletotrichum falcatum, Sclerotium rolfsii, Alternaria solani, Penicillium sp., Pythium aphanidermatum, Phytophthora palmivora, Curvularia lunata, Sclerotinia sclerotiorum, and Aspergillus niger under in vitro conditions as follows: Four single bacterial colonies on NA plates were streaked around the edge of a 90-mm diameter petri plate and the plates were incubated at 28° C. for two days. An agar plug inoculum of the fungus to be tested (5-mm square) was then transferred to the center of the plate individually from a source plate of the fungus. After incubation for 5 to 7 days inhibition zones were readily observed in the case of bacterial strains having biocontrol activity, as the fungal growth around the streak was inhibited. In case of ...

example 3

Screening of Bacterial Strains for Ability to Promote Plant Growth in Greenhouse

[0159] The 150 bacterial strains that were suppressive to pathogenic fungi in vitro were screened in a greenhouse by growing bacteria treated maize seeds in non-sterile soil and comparing the treated maize with control maize plants grown without bacterial treatment.

[0160] The process of screening of bacterial strains for ability to promote plant growth in a greenhouse of the present invention is disclosed with particular reference to the plant maize. However it should not be inferred that the process of screening of bacterial strains for ability to promote plant growth in greenhouse is restricted to this plant, as any suitable other plant may be employed.

[0161] Non-sterile field soil from the farm of National Botanical Research Institute, Lucknow was used to evaluate the plant growth promotion potential of the 150 strains in greenhouse.

[0162] A bacterial inoculum for maize seeds was prepared by scra...

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Abstract

A synergistic composition useful as bioinoculant includes bacterial strains of accession Nos. NRRL B-30486, NRRL B-30487, and NRRL-B 30488, individually or in all possible combinations, and optionally a carrier, with each of the strains showing plant promoting activity, phytopathogenic fungi controlling activity, abiotic stress conditions tolerating capability, phosphate solubilization capability under abiotic stress conditions; and further, a method of producing the composition thereof, and in addition, a method of isolating the bacterial strains from cow ‘Sahiwal’.

Description

[0001] This application is a divisional application of U.S. Ser. No. 10 / 173,745, filed on Jun. 19, 2002, allowed on Sep. 20, 2005, which is a non-provisional application of U.S. Ser. No. 60 / 316,283, filed on Sep. 4, 2001, the entire contents of both applications are incorporated herein by reference and priority is claimed to each.BACKGROUND OF THE INVENTION [0002] 1. Field of the Invention [0003] The present invention relates to a synergistic composition useful as a bioinoculant, said composition comprising bacterial strains of accession Nos. NRRL B-30486, NRRL B-30487, and NRRL-B 30488, individually or in all possible combinations, and optionally a carrier, with each of the strains showing plant growth-promoting activity, phytopathogenic fungi controlling activity, abiotic stress conditions tolerating capability and phosphate solubilization capability under abiotic stress conditions. The invention further relates to a method of producing said composition thereof, and in addition, t...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A01N63/00C12N1/20A01N63/22C05F11/08
CPCA01N63/00C05F11/08A01N2300/00Y02W30/40A01N63/22
Inventor NAUTIYAL, CHANDRA SHEKHARMEHTA, SANGEETASINGH, HARIKESH BAHAADURPUSHPANGADAN, PALPU
Owner NAUTIYAL CHANDRA SHEKHAR
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