Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Nucleotide sequences mediating male fertility and method of using same

a technology of nucleotide sequences and male fertility, applied in the field of nucleotide sequences mediating male fertility and using same, can solve the problems of few satisfactory efforts to establish, and the efforts to establish have been mad

Inactive Publication Date: 2006-09-21
PIONEER HI BRED INT INC
View PDF8 Cites 19 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patent is about identifying specific parts of DNA that are important for male fertility in plants. These parts are called promoters, and they help control when and how the DNA is expressed. The patent also describes using these DNA parts to improve plant fertility.

Problems solved by technology

Some efforts have been made to establish the mechanism of sterility in maize, but few have been satisfactory.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nucleotide sequences mediating male fertility and method of using same
  • Nucleotide sequences mediating male fertility and method of using same
  • Nucleotide sequences mediating male fertility and method of using same

Examples

Experimental program
Comparison scheme
Effect test

example 2

Library Construction and Screening

[0072] The process of cDNA library screenings is commonly known among those skilled in the art and is described at Sambrook, J., Fritsch, E. F., Maniatis T., et al., Molecular Cloning: A Laboratorv Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor Lab Press, Plainview, N.Y. (1989). Libraries were created as follows.

[0073] DNA from a sterile plant was digested with EcoRI and run on a preparative gel. DNA with a molecular weight between 5.0 and 6.0 Kb was excised from the gel, electroeluted and ethanol precipitated. This DNA was ligated into the Lambda Zap vector (Stratagene™) using the manufacturer's protocol. The ligated DNA was packaged into phage particles using Gigapack Gold (Stratagene™). Approximately 500,000 PFU were plated and lifted onto nitrocellulose membranes. Membranes were hybridized with the Mu8 probe. A pure clone was obtained after 3 rounds of screening. The insert was excised from the phage as a plasmid and designated SBMu...

example 3

Expression Analysis and cDNA Isolation

[0074] Northern analysis can be used to detect expression of genes characteristic of anther development at various states of microsporogenesis. Northern analysis is also a commonly used technique known to those skilled in the art and is similar to Southern analysis except that MRNA rather than DNA is isolated and placed on the gel. The RNA is then hybridzed with the labeled probe. Potter, E., et al., “Thyrotrotropin Releasing Hormone Exerts Rapid Nuclear Effects to Increase Production of the Primary Prolactin in RNA Transcript,”Proc. Nat. Acad. Sci. USA 78:6662-6666 (1981), Lechelt, et al., “Isolation & Molecular Analysis of the Plows,”Mol. Gen. Genet. 219:225-234 (1989). The PstI fragment from the SBMu200-3.1 clone was used to probe a Northern blot containing kernel, immature ear, seedling and tassel RNA. A signal was seen only in tassel RNA at approximately the quartet stage of microsporogenesis, as reflected in FIG. 3. The transcript is abou...

example 4

Sequence Analysis

[0075] The SBMu200-3.1 genomic clone and the cDNA clone, SBMu200-8.1, were sequenced by Loftstrand Labs Limited. Sanger, F., Nicklen, S., Coulson A. R. (1977) “DNA sequencing with chain terminating inhibitors”Proc. Natl. Acad. Sci. USA 74:5463-5467. The sequences are set forth in FIGS. 4 and 5 and the comparison is at FIG. 6. The cDNA / genomic comparison reveals five introns are present in the genomic clone. The Mu8 insertion occurs in exon 1. Testing for codon preference and non-randomness in the third position of each codon was consistent with the major ORF in the cDNA being the likely protein-coding ORF. There is a putative Met start codon at position 1089 in the genomic clone. The cDNA homology with respect to the genomic clone begins at nucleotide 1094. Thus SBMu200-8.1 does not represent a full length clone and lacks 5 bases up to the putative Met start codon. A database search revealed significant homology to P450 enzymes found in yeast, plants and mammals. P...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Tmaaaaaaaaaa
resistanceaaaaaaaaaa
heataaaaaaaaaa
Login to View More

Abstract

Nucleotide sequences mediating male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences are useful in mediating male fertility in plants.

Description

[0001] This application is a continuation of previously filed and co-pending application U.S. Ser. No. 10 / 412,000, filed Apr. 11, 2003, which is a continuation of previously filed and application U.S. Ser. No. 09 / 670,153, filed Sep. 26, 2000, now abandoned.BACKGROUND OF THE INVENTION [0002] Development of hybrid plant breeding has made possible considerable advances in quality and quantity of crops produced. Increased yield and combination of desirable characteristics, such as resistance to disease and insects, heat and drought tolerance, along with variations in plant composition are all possible because of hybridization procedures. These procedures frequently rely heavily on providing for a male parent contributing pollen to a female parent to produce the resulting hybrid. [0003] Field crops are bred through techniques that take advantage of the plant's method of pollination. A plant is self-pollinating if pollen from one flower is transferred to the same or another flower of the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(United States)
IPC IPC(8): A01H5/00C07H21/04C12N15/82C12N5/04A01H1/00C07K14/415C12N15/29
CPCC07K14/415C12N15/8217C12N15/8222C12N15/8231C12N15/8289
Inventor ALBERTSEN, MARCFOX, TIMHUFFMAN, GARYTRIMNELL, MARY
Owner PIONEER HI BRED INT INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com