Rumen fermentation improving agent

a technology of rumen fermentation and improving agent, which is applied in the direction of antibacterial agents, drug compositions, metabolic disorders, etc., can solve the problems of loss of energy efficiency, social problems, and inability to use technology, etc., to improve the fermentation rate of rumen, promote the growth of ruminants, and improve feed efficiency

Inactive Publication Date: 2010-07-22
HOKKAIDO UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0016]An object of the present invention is to promote the growth of a ruminant and to improve feed efficiency by improving the rumen fermentation in the ruminant and contributing to suppressing the generation of greenhouse gas.
[0017]The inventors of the present invention have intensively studied in order to solve the above problems, and as a result, the inventors have found that a feed containing cashew nut shell liquid suppresses methane production and promotes propionic acid production in a rumen. Thus, the present invention has been accomplished.

Problems solved by technology

Therefore, it is a loss of energy efficiency when methane is generated from the rumens.
On the other hand, as a result of adding antibiotics to a feed, the antibiotics are distributed widely in the environment, and accordingly, an appearance of antibiotic-resistant bacteria has become a social problem.
However, those technologies are not in practical use yet, because there are problems in that their effects are not stable, the registration thereof as feeds is not permitted, and the like.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

Effects of Cashew Nut Shell Liquid (CNSL) on Gas Production and Production of Volatile Fatty Acids In Vitro

(1) Sample

[0048]Cashew nut shell liquid (CNSL), which was extracted by compressing cashew nut shells, was obtained from Cashew Trading Co., Ltd. For a culture inoculum, there was used rumen juice (quadruple gauze filtrate) collected from a Holstein cow (fitted with rumen cannula) owned by Experiment Farm, Field Science Center for Northern Biosphere, Hokkaido University. The inoculum was diluted two fold with McDougal's artificial saliva (pH 6.8) and used.

(2) Culture

[0049]The culture was performed in such a manner that the concentration of CNSL in a test culture medium was 500 mg / L. 0.05 g of CNSL was dissolved in 1 ml of ethanol, and 100 μl of the solution was added to a Hungate tube. Ethanol was volatilized by leaving the resultant standing for several hours. Then, as a culture substrate, added therein were 0.15 g of corn starch, 0.025 g of blended feed powder, and 0.025 g of ...

example 2

Antibacterial Action of CNSL

[0059]For examining the antibacterial action of CNSL, the following strains were each cultured in a brain-heart-infusion medium (manufactured by NISSUI PHARMACEUTICAL CO., LTD.) at 37° C. for a day: Staphylococcos aureus strain isolated from a bovine; Streptococcus bovis DSM20065 strain; Bacillus subtilis NBRC3009 strain; Escherichia coli ATCC11303 strain; Pseudomonas aeruginosa NBRC12689 strain; and Saccharomyces cerevisiae NBRC10217 strain. Into the brain-heart-infusion medium to which CNSL was added, 10 μL each of culture media of the above-mentioned strains were inoculated, and the resultant was cultured at 37° C. for two days, to thereby calculate a minimum growth-inhibitory concentration (MIC).

[0060]Table 3 shows the results.

TABLE 3MIC(μg / ml)Gram-positiveStaphylococcos aureus isolated from6.25bacteriaa bovineStreptococcus bovis DSM200659.38Bacillus subtilis NBRC30096.25Gram-negativeEscherichia coli ATCC11303>1600bacteriaPseudomonas aeruginosa NBRC12...

example 3

Effects of CNSL Concentration on Production of Methane and Volatile Fatty Acids In Vitro

(1) Sample

[0062]Cashew nut shell liquid (CNSL), which was extracted by compressing cashew nut shells, was obtained from Cashew Trading Co., Ltd. For a culture inoculum, there was used rumen juice (quadruple gauze filtrate) collected from a Holstein cow (fitted with rumen cannula) owned by Experiment Farm, Field Science Center for Northern Biosphere, Hokkaido University. The inoculum was diluted two fold with McDougal's artificial saliva (pH 6.8) and used.

(2) Culture

[0063]The culture was performed in such a manner that the concentrations of CNSL in test culture media were 50, 100, 250, and 500 mg / L. CNSL was dissolved in 1 ml of ethanol, and 100 ml of the solution was added to a Hungate tube. Ethanol was volatilized by leaving the resultant standing for several hours. Then, as a culture substrate, added therein were 0.15 g of corn starch, 0.025 g of blended feed powder, and 0.025 g of powdered orc...

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PUM

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Abstract

The present invention provides a rumen fermentation improving agent, comprising cashew nut shell liquid.

Description

TECHNICAL FIELD[0001]The present invention relates to a rumen fermentation improving agent, a composition for a feed, and a feed each containing cashew nut shell liquid, and a breeding method for a ruminant or the like using the same.BACKGROUND ART[0002]In ruminants such as cattle and sheep, a feed is digested and fermented by microorganisms in their rumens, and the ruminants live by utilizing the fermentation products. Therefore, it is a loss of energy efficiency when methane is generated from the rumens. Further, because methane is a greenhouse gas that influences the global warming, it is important to reduce the amount of methane produced in the rumens of the ruminants.[0003]A methanogen in a rumen reduces carbon dioxide by utilizing hydrogen to thereby produce methane. The contribution ratio of methane to the global warming is second highest after carbon dioxide, and it is considered that methane emitted from ruminants accounts for 15 to 20% of the total emission of methane.[000...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K36/22A61P1/14A01K21/00A23L25/00A61K36/00A61K131/00A61P3/02A61P31/04
CPCA23K20/158A61K36/22A61P1/14A61P3/00A61P3/02A61P31/04
Inventor KOBAYASHI, YASUONAGASHIMA, KYOMOCHIZUKI, MASAMI
Owner HOKKAIDO UNIVERSITY
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