Antiviral composition comprising a sulfated polysaccharide

a technology of sulfated polysaccharides and compositions, applied in the field of sulfated polysaccharides, can solve the problems of inability to uniformly infect the immune system, the mechanism of sulphated polysaccharides, especially carrageenans, and the inability to inhibit viral replication

Inactive Publication Date: 2011-03-10
MARINOMED BIOTECHNOLOGIE GMBH
View PDF8 Cites 17 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

RSV and parainfluenza virus infections may cause serious respiratory infections in infants and young children but may also cause severe disease in elderly persons and adults having an impaired immune system.
The mechanism by which sulphated polysaccharides, particularly the carrageenans, inhibit viral replication and infectivity may not be uniform as different investigators reported contradictory findings when working with different viruses and cell types.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Antiviral composition comprising a sulfated polysaccharide
  • Antiviral composition comprising a sulfated polysaccharide
  • Antiviral composition comprising a sulfated polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

example 1

Effect of Different Concentrations of Iota-Carrageenan on Influenza A Virus Plaque Formation in MDCK Cells

[0112]Virus suspensions containing 60-80 pfu of influenza virus A / Chile / 1 / 93 H1N1 were mixed with a iota-carrageenan stock solution to final concentrations of 0.1, 1, 10, 25, 50 or 100 μg / ml. Confluent monolayers of the canine kidney cell line MDCK in six well plates were infected with the virus suspensions for 60 min at 34° C. The infection inoculum was removed and cells were washed with PBS and agarose overlay containing 0.6% agarose was added. Plates were incubated at 36° C. in a humidified atmosphere of 5% CO2 in air. 48-60 h after infection the agarose overlay was removed, cells were stained with crystal violet stain and visible plaques were counted. The percentage of plaque formation relative to the infected control (without iota-carrageenan treatment) was determined for each iota-carrageenan concentration.

[0113]As shown in FIG. 1, it was found that iota-carrageenan inhibi...

example 2

Effect of Different Concentrations of Iota-Carrageenan on Influenza A Virus Plaque Formation in MDCK Cells

[0114]Virus suspensions containing 60-80 pfu of influenza virus A / Aichi2 / 68H3N2 were mixed with a iota-carrageenan stock solution to final concentrations of 75, 150 or 300 μg / ml. Confluent monolayers of the canine kidney cell line MDCK in six well plates were infected with the virus suspensions for 60 min at 34° C. The infection inoculum was removed and cells were washed with PBS and agarose overlay containing 0.6% agarose was added. Plates were incubated at 37° C. in a humidified atmosphere of 5% CO2 in air. 48-60 h after infection the agarose overlay was removed, cells were stained with crystal violet stain and visible plaques were counted. The percentage of plaque formation relative to the infected control (without iota-carrageenan) was determined for each iota-carrageenan concentration.

[0115]As shown in FIG. 2, it was found that iota-carrageenan inhibits, in a dose dependent...

example 3

Effect of Different Concentrations of Iota-Carrageenan on Parainfluenza Virus 3 Plaque Formation in Hep-2 Cells

[0116]Virus suspensions containing 60-80 pfu of parainfluenza virus 3 were mixed with a iota-carrageenan stock solution to final concentrations of 0.1, 1, 10, 25, 50 and 100 μg / ml. The mixture was incubated for 1 h at 34° C. Confluent monolayers of Hep-2 cells in six well plates were infected with the virus suspensions for 60 minutes a 34° C. The infection inoculum was removed and cells were washed with PBS and agarose overlay containing 0.6% agarose was added. The trays were incubated in a humidified, 5% CO2 atmosphere. 48-60 h after infection the agraose overlay was removed, cells were stained with crystalviolett stain and visible plaques were counted. The percentage of plaque formation relative to the infected control (without iota-carrageenan) plates was determined for each iota-carrageenan concentration.

[0117]As shown in FIG. 3, it was found that iota-carrageenan inhib...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
average molecular weightaaaaaaaaaa
average molecular weightaaaaaaaaaa
molecular weightsaaaaaaaaaa
Login to view more

Abstract

The present invention provides for the use of iota- and/or kappa-carrageenan for the manufacture of an antiviral pharmaceutical composition for the prophylaxis or treatment of a pathological condition or disease caused by or associated with an infection by a respiratory virus selected from the group consisting of orthomyxovirus, paramyxovirus, adenovirus and coronavirus. The present invention further provides for the use of fucoidan, in particular of high molecular weight fucoidan, for the manufacture of an antiviral pharmaceutical composition for the prophylaxis or treatment of a pathological condition or disease caused by or associated with an infection by a respiratory virus selected from the group consisting of orthomyxovirus and paramyxovirus.

Description

TECHNICAL FIELD[0001]The present invention relates to sulfated polysaccharides selected from the group of carrageenans and fucoidans and pharmaceutical compositions made thereof, wherein said sulfated polysaccharides are present as antiviral active ingredients, for medical or veterinary use in the prevention or treatment of diseases caused by or associated with a virus entering an individual's body via the respiratory tract, the virus being selected from the group of orthomyxoviridae, paramyxoviridae, adenoviridae and coronaviridae.BACKGROUND OF THE INVENTION[0002]Orthomyxoviridae are RNA viruses, the most prominent members being influenza virus species. Influenza A and B virus are the two types of influenza viruses that cause epidemic human disease. They are typically spread from person to person, primarily through respiratory droplet transmission.[0003]The paramyxoviridae family includes respiratory syncytial virus (RSV), parainfluenza virus and metapneumavirus. RSV and parainflue...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(United States)
IPC IPC(8): A61K31/731C07H5/10A61P31/16A61P31/20A61P11/06A61P29/00A61P37/08A61P37/04
CPCA61K31/737A61K31/731A61P11/00A61P11/06A61P11/08A61P29/00A61P31/00A61P31/12A61P31/14A61P31/16A61P31/20A61P37/04A61P37/08
Inventor GRASSAUER, ANDREASPRIESCHL-GRASSAUER, EVA
Owner MARINOMED BIOTECHNOLOGIE GMBH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap