Hyaluronic acid cryogel - compositions, uses, processes for manufacturing

a technology of hyaluronic acid and cryogel, which is applied in the field of hyaluronic acid cryogel, can solve the problems of unable to recover the pore structure after hydrogel formation, the effect of ha injection, and the difficulty of hydrogel formation recovery

Inactive Publication Date: 2011-10-27
BURDICA BIOMED
View PDF2 Cites 22 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, some placebo controlled studies have cast doubt on the efficacy of HA injections, and HA is recommended primarily as a last alternative to surgery.
Proteins, such as collagen or fibrin, and polysaccharidic materials, like chitosan or glycosaminoglycans (GAGs), have all proved suitable in terms of cell compatibility, but some issues with potential immunogenicity still remain.
However, due to the nature of the softness of the material, it is difficult to recover the pore structure after hydrogel formation.
It is already well known that HA is not thermo-stable and easy to degrade at acidic or alkaline conditions at high temperature; conditions which are commonly used for chemical modification of HA.
This will significantly change the yield of production of HA derivatives, and the biocompatibility of the finished products, due to the generation of HA degradation fragments.
However, it is difficult (due to the high molecule weight of HA) to achieve successful crosslinking to create a well organized crosslinked network.
Additionally, further purification to remove the toxic reactant residue is also problematic.
None of these methods include the step of adding a cross-linking agent.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Hyaluronic acid cryogel - compositions, uses, processes for manufacturing
  • Hyaluronic acid cryogel - compositions, uses, processes for manufacturing
  • Hyaluronic acid cryogel - compositions, uses, processes for manufacturing

Examples

Experimental program
Comparison scheme
Effect test

example 1

Preparation of the HA Cryogel

[0168]The solution of HA (MW 6000) (10 g), sodium hydroxide (2 g) in 60 ml of DMF / H2O (40 / 20) was degassed and after addition of 1 ml of BDDE and have a good mixing was slowly poured into a glass tube. The polymer solution in the tube was frozen and kept at −18° C. overnight. Then the tube was thawed at IPA and a spongy cryogel thus formed was washed thoroughly with IPA to remove the impurities and vacuum dried to obtain HA cryogel.

example 2

Preparation of the HA Cryogel

[0169]The solution of HA (MW 20,000) (10 g)), sodium hydroxide (2 g) in 60 ml water was degassed and after addition of 1 ml of BDDE and have a good mixing was slowly poured into a glass tube. The polymer solution in the tube was frozen and kept at −18° C. overnight. Then the tube was thawed at IPA and a spongy cryogel thus formed was washed thoroughly with IPA to remove the impurities and vacuum dried to obtain HA cryogel.

example 3

Preparation of the HA Cryogel

[0170]The solution of HA (MW 1,000,000) (10 g)), sodium hydroxide (2 g) in 60 ml water was degassed and after addition of 1 ml of BDDE and have a good mixing was slowly poured into a glass tube. The polymer solution in the tube was frozen and kept at −18° C. overnight. Then the tube was thawed and a spongy cryogel thus formed was washed thoroughly with IPA to remove the impurities and vacuum dried to obtain HA cryogel.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
temperatureaaaaaaaaaa
molecular weightaaaaaaaaaa
molecular weightaaaaaaaaaa
Login to view more

Abstract

There is provided a process for preparing a HA cryogel, the process comprising the steps of combining HA, a cross-linking agent and a solvent to form a solution, freezing the solution before the formation of less than 10% of the cross-linking bonds of the cross-linked HA cryogel formed and thawing the solution. An HA cryogel is also provided, in particular an HA cryogel obtained from this process.

Description

FIELD OF INVENTION[0001]The present invention relates to a hyaluronic acid cryogel, its preparation and its use. More particularly, the invention relates to a hyaluronic acid cryogel as an implant in the field of biomedical engineering, the preparation of such a cryogel, and the use of said cryogel.BACKGROUND OF THE INVENTION[0002]Hyaluronic acid (also known as hyaluronan or sodium hyaluronate, and abbreviated to as HA) is a non-sulfated glycosaminoglycan which is distributed widely throughout connective, epithelial, and neural tissues. It is one of the main components of the extracellular matrix, contributes significantly to cell proliferation and migration, and may also be involved in the progression of some malignant tumors.[0003]For example, HA is a major component of the synovial fluid and has been found to increase the viscosity of the fluid. Along with lubricin, it is one of the fluid's main lubricating components. HA is an important component of articular cartilage, where it...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(United States)
IPC IPC(8): A61K31/738A61Q19/08A61P17/02A61K9/00A61K8/73
CPCA61K9/0021A61K31/728A61K9/06A61P17/02
Inventor ZHAO, XIAOBIN
Owner BURDICA BIOMED
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap