Binary DNA Probe for Fluorescent Analysis of Nucleic Acids

a nucleic acid and fluorescent analysis technology, applied in the field ofbinary probes for fluorescent analysis of nucleic acids, can solve the problems of inability to select and use known methods, and inability to detect nucleotide hybrid duplexes in living cells, etc., to achieve more stable results
US20140057252A1Inactive Publication Date: 2014-02-27THE TRUSTEES OF COLUMBIA UNIV IN THE CITY OF NEW YORK
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
THE TRUSTEES OF COLUMBIA UNIV IN THE CITY OF NEW YORK
Publication Date
2014-02-27
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention is directed to binary oligonucleotide probes for nucleic analysis, which probes can be made of DNA or RNA that recognize nucleic acid analytes (both DNA and RNA) with unprecedented high selectivity under mild conditions and are highly sensitive to single nucleotide mismatches (SNP single nucleotide polymorphisms) without PCR amplification. In one group, the binary probes indicate that they have hybridized to a particular nucleic analyte by binding to a molecular beacon that gives off a fluorescent signal. A second group of binary probes bind to a dye such as malachite green, where upon hybridization to analyte the fluorescence of the dye increases dramatically and is easily detected and measured. The new binary probes require only about five minutes at room temperature to generate a detectable signal.
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Description

CROSS REFERENCE TO RELATED APPLICATIONS

[0001] This application claims benefit of Provisional Application 60 / 793,481, filed on Apr. 20, 2006, and Provisional Application 60 / 788,029, filed on Mar. 31, 2006, which are incorporated herein by reference, under 35 U.S.C. §119(e).STATEMENT OF GOVERNMENTAL INTEREST

[0002] This invention was made with Government support under Contract Nos. RO1 EB000675 awarded by National Institute of Biomedical Imaging and Bioengineering of National Institute of Health, R21HG004060 awarded by National Human Genome Research Institute of National Institutes of Health and BES 0321972 awarded by National Science Foundation. The Government has certain rights in the invention.BACKGROUND OF THE INVENTION

[0003] 1. Field of the Invention

[0004] The present invention relates to binary probes for fluorescent analysis of nucleic acids using molecular beacons or dyes.

[0005] 2. Description of the Related Art

[0006] Sequence-specific detection of nucleic acids is crucial to disease...

Claims

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