Compositions comprising sulforaphane or a sulforaphane precursor and a milk thistle extract or powder

a technology of sulforaphane and precursor, which is applied in the direction of drug compositions, peptide/protein ingredients, metabolic disorders, etc., can solve the problems of not all animals are capable of achieving nafld surgical or pharmacological treatment, patients are susceptible to oxidative stress, illness, cancer, etc., to reduce secondary recurrence, reduce symptoms, and prevent the effect of nafld

Inactive Publication Date: 2015-04-30
NUTRAMAX LABORATORIES INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0017]The present invention provides a composition comprising: (i) sulforaphane or a derivative thereof, and (ii) a milk thistle extract or powder. The present invention also provides a method of treating, preventing, reducing the occurrence of, decreasing the symptoms associated with, and / or reducing secondary recurrences of, a disease or condition associated with the liver, prostate, brain, lung, kidneys, colon, breast, esophagus, pancreas, or ovaries in a subject, comprising administering to the subject: (i) sulforaphane or a derivative thereof, and (ii) a milk thistle extract or powder. The present invention also provides a method of increasing glutathione levels in a subject in need thereof in a subject, comprising administering to the subject, comprising administering to the subject: (i) sulforaphane or a derivative thereof, and (ii) a milk thistle extract or powder. The present invention also provides a method of treating, preventing, reducing the occurrence of, decreasing the symptoms associated with, and / or reducing secondary recurrences of a non-alcoholic fatty liver disease (NAFLD) and / or any other disorder of the liver, comprising administering to the subject: (i) sulforaphane or a derivative thereof, and (ii) a milk thistle extract or powder.
[0018]The present invention provides a composition comprising: (i) a broccoli extract or powder, and (ii) milk thistle extract or powder. The present invention also provides a method of treating, preventing, reducing the occurrence of, decreasing the symptoms associated with, and / or reducing secondary recurrences of, a disease or condition associated with the liver, prostate, brain, lung, kidneys, colon, breast, esophagus, pancreas, or ovaries in a subject, comprising administering to the subject: (i) a broccoli extract or powder, and (ii) a milk thistle extract or powder. The present invention also provides a method of increasing glutathione levels in a subject in need thereof in a subject, comprising administering to the subject, comprising administering to the subject: (i) a broccoli extract or powder, and (ii) a milk thistle extract or powder. The present invention also provides a method of treating, preventing, reducing the occurrence of, decreasing the symptoms associated with, and / or reducing secondary recurrences of a non-alcoholic fatty liver disease (NAFLD) and / or any other disorder of the liver, comprising administering to the subject: (i) a broccoli extract or powder, and (ii) a milk thistle extract or powder.

Problems solved by technology

There are currently no surgical or pharmacological treatments for NAFLD.
Low levels of glutathione can cause a patient to be susceptible to oxidative stress, illness, and cancer.
However, upon ingestion of glucoraphanin, not all animals are capable of achieving its conversion to sulforaphane, most likely due to variations in microflora populations and overall health.

Method used

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  • Compositions comprising sulforaphane or a sulforaphane precursor and a milk thistle extract or powder
  • Compositions comprising sulforaphane or a sulforaphane precursor and a milk thistle extract or powder
  • Compositions comprising sulforaphane or a sulforaphane precursor and a milk thistle extract or powder

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0072]The following is an exemplary formulation:

Glucoraphanin-containing broccoli extract (about 12% w / w), 50 mg to 5 g

Myrosinase-containing freeze-dried broccoli sprout powder, 25 mg to 500 mg

Ascorbic acid, 5 mg to 500 mg

Milk thistle extract (about 20% to 35% w / w silibinin), 25 mg to 5 g

example 2

[0073]A Hydrophobic Interaction Chromatographic (HILIC) method was developed, comprising the following conditions:

Column: Waters BEH Amide, 1.7-μm particle size; 2.1 mm×100 mm

Mobile Phase: 20% 10 mM Ammonium Acetate, pH 5.0; 80% Acetonitrile;

[0074]Separation mode: isocratic

Column Temperature: 70° C.

[0075]Flow Rate: 0.7 mL / min

The above conditions allow separation of five typical Brassicaceae glucosinolates, including the sulforaphane precursor, glucoraphanin.

example 3

Consumption of Glucoraphanin as a Function of the Ascorbic Acid Concentration

[0076]About 250 mg of broccoli seed extract containing about 12% (w / w) glucoraphanin were subjected to hydrolysis by a fixed concentration of broccoli sprout-derived myrosinase in the presence of variable concentration of ascorbic acid, ranging from 0 to 600 μmoles / Liter. The reaction mixtures were thermostated at 38° C.; aliquots were withdrawn every 15 minutes for 60 minutes, and concentration of glucoraphanin determined chromatographically. The rate of glucoraphanin consumption was interpreted as the rate its conversion to sulforaphane. Graphical representation of glucoraphanin content reduction as a function of increasing ascorbic acid concentration results in a series of linear plots; the slopes of the linear regression lines reflect the rate of glucoraphanin consumption, in μmoles / minute. It is apparent that in the presence of 600 μmoles / Liter concentration of ascorbic acid, the reaction rate increase...

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Abstract

The invention relates to the combination of a sulforaphane precursor, an enzyme capable of converting the sulforaphane precursor to sulforaphane, an enzyme potentiator, and a milk thistle extract or powder. The invention also relates to the combination of a sulforaphane or a derivative thereof and a milk thistle extract or powder. The invention also relates to the combination of a broccoli extract or powder and a milk thistle extract or powder. The invention provides compositions and methods relating to these combinations.

Description

[0001]This application claims priority to the following applications, each of which is incorporated by reference in its entirety: U.S. Provisional Patent Application No. 61 / 668,328, filed on Jul. 5, 2012; U.S. Provisional Patent Application No. 61 / 668,342, filed on Jul. 5, 2012; U.S. Provisional Patent Application No. 61 / 668,386, filed on Jul. 5, 2012; U.S. Provisional Patent Application No. 61 / 668,396, filed on Jul. 5, 2012; U.S. Provisional Patent Application No. 61 / 668,364, filed on Jul. 5, 2012; U.S. Provisional Patent Application No. 61,668,374, filed on Jul. 5, 2012; and U.S. Provisional Patent Application No. 61 / 794,417, filed on Mar. 15, 2013.FIELD OF THE INVENTION[0002]The present invention relates to the combination of a sulforaphane precursor, an enzyme capable of converting the sulforaphane precursor to sulforaphane, an enzyme potentiator, and a milk thistle extract or powder. The present invention also relates to the combination of a sulforaphane or a derivative thereof...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K38/47A61K36/31A61K9/28A61K36/28A61K31/7028A61K31/375
CPCA61K38/47A61K31/7028A61K31/375C12Y302/01147A61K36/28A61K36/31A61K9/28A61K9/0053A61K31/26A61K36/06A61K36/07A61K45/06A61K2300/00A61K33/06A23L33/105A61K9/14A61P3/06A61K31/194A61K31/19A61K31/716A61K31/357A61K31/275A61P35/00A61K31/095A61K47/42A23V2002/00
Inventor CORNBLATT, BRIANCORNBLATT, GRACEBZHELYANSKY, ANTONHENDERSON, ROBERT
Owner NUTRAMAX LABORATORIES INC
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