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Method of Myceliating Coffee

a technology of myceliation and coffee, applied in the field of methods, can solve the problems of time-consuming fruiting of mushrooms, long time-consuming medicinal or gourmet mushrooms, etc., and achieve the effects of enhancing the neuroprotective effect of products, enhancing production efficiency, and improving myceliation

Inactive Publication Date: 2015-10-01
MYCOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patent is about a method for growing mushroom mycelia in a three-dimensional environment using small spherical conglomerations. This method accelerates the growth of hyphae and allows for continuous harvest of the liquid media. The resulting mushroom substrate has interstitial spaces between the substrate elements, which improves myceliation. The substrate is coated with a mixture of vegetable oil and lecithin, which enhances the neuro-protective effects of the mushroom substrate. The patent also describes a liquid state fermentation method that can be performed in an undisturbed environment. The resulting mushroom product has been found to have neuro-regeneration and neuro-protection properties. In one embodiment, the use of a specific mushroom species, Hericium erinaceus, is described.

Problems solved by technology

However, fruiting mushrooms can be time consuming.
Most medicinal and or gourmet mushrooms take a long time to come to fruition.
In contrast, plant cell walls are made of cellulose polysaccharides whose (1->4) b-glycosidic glucose linkages are likewise difficult to digest by our enzymes; however, as the cell walls of fungi are primarily composed of (1->3) b-glycosidic linkages, with (1->6) linked side chains, they may be broken down by minimal processing using water, heat and mechanical treatment into smaller, more easily digestible, immunologically-active polysaccharide molecules of variable microparticulate size called b-glucans, and related glycoprotein compounds.

Method used

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  • Method of Myceliating Coffee
  • Method of Myceliating Coffee

Examples

Experimental program
Comparison scheme
Effect test

process example # 1

PROCESS EXAMPLE #1

[0056]Using specific and pure strains of fungi obtained from referenced collections are manipulated in sterile environments at 68 degrees F. to 90 degrees F., in 1 gal to 10 gal plastic bags, 1 qt to 1 gal glass jar, or on 10 cm to 15 cm Petri plates, using undefined, organic fruit and vegetable-based media with 1.5% agar (w / v), in order to monitor and ensure the general vigor and health of strains. Mycelial samples are grown in a gentle, ambient sterile airflow for 2 to 4 weeks, then excised from Petri plates and subsequently used for inoculation into liquid-state fermentation employing a similar undefined fruit and vegetable-based media (but with no agar), using ambient air, in 1 qt to 1 gal glass jars, with agitated or unagitated cultures in ambient air in stainless steel tanks which have been designed for commercial beer brewing and / or fermentation. There is a subtle difference between unagitated and agitated liquid-state fermentation. Unagitated liquid-state f...

process example # 2

PROCESS EXAMPLE #2

[0057]In using unagitated liquid state fermentation, after commanding the liquid-state medium for a growth period of 2 to 4 weeks, cultures form a floating mass of hyphae, which are gently blended with a sharp, sterile cutting device prior to being used for inoculation. Gentle blending is achieved by mixing or low homogenization in a commercial blender in short bursts at slow speeds. Aliquots of blended liquid-state culture are used to inoculate sterilized unprocessed fruits and or vegetables, cereal grains, and / or culinary seed, or pasteurized culinary spice, medicinal herbs, natural flavorings, tea mixes, green vanilla beans, green cacao beans, and / or green coffee beans.

SUBSTRATE TREATMENT

[0058]Agricultural substrate requiring sterilization such as unprocessed fruits and / or vegetables, cereal grains or culinary seed, is modified using heat treatment by pressure cooking for 90 minutes at 15 lb / in2 and then cooled to room temperature prior to inoculation by liquid-...

process example # 3

PROCESS EXAMPLE #3

[0059]In one embodiment: small batch liquid-state and solid-state fermentation; pure cultures of fungi are grown semi-aerobically and inoculated into 1 qt to 1 gal glass jars, or into 1 gal to 5 gal autoclavable plastic bags containing properly prepared grain or similar agricultural materials such as fruit, vegetables, herbs, spices, teas, green vanilla beans, green cacao beans, and / or green coffee beans; then, depending upon strain of fungi and substrate, grown at 70 degrees F. to 90 degrees F. Cultures in jars or bags are gently mixed every few days until they command the substrate whereby eventually becoming somewhat resistant to mixing or shaking, usually 2 to 4 weeks depending upon strain, and are intended for small-scale production of functional food products sold in a tempeh form. The myceliated green vanilla beans are cooked or baked; the myceliated green cacao beans are bake or toasted; and the myceliated green coffee beans are toasted or roasted. Myceliat...

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Abstract

The present invention includes a method of myceliating coffee beans including sterilizing the coffee beans, preparing a liquid tissue culture including an aliquot of fungal liquid tissue culture derived from liquid state fermentation, and inoculating the coffee beans with the fungal liquid tissue culture. Next, the step of enabling mycelium growth on the coffee includes controlling temperature, humidity, and sterility of the environment. The aliquot of fungal liquid tissue culture is optionally agitated to form hyphael conglomerations of mycelium having sizes of less than 1 mm in diameter.

Description

RELATED APPLICATIONS[0001]This application is a continuation of U.S. patent application Ser. No. 13 / 844,685, filed Mar. 15, 2013, entitled “Method for myceliating coffee” which application claims the benefit of U.S. Provisional Application No. 61 / 697,506, filed Sep. 6, 2012, which is incorporated herein by reference in its entirety.FIELD OF THE INVENTION[0002]This invention pertains to methods of liquid-state and solid-state myceliation of agricultural materials into a range of potent nutraceutical and delicious functional foods for humans.BACKGROUND AND SUMMARY OF THE INVENTION[0003]Tempeh is a soy-based product that typically utilizes Rhizopus oligosporus to ferment soybeans. R. oligosporus is also used to ferment other legumes.[0004]The present invention utilizes mycelium from select, referenced strains of Basidiomycota and Ascomycota fungi grown in liquid-state culture to solid-state ferment agricultural materials containing or admixed with plant, vegetable and or fungi biomass,...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A23F5/02A23L7/104A23L11/00A23L21/25A23L23/00A23L33/00
CPCA23F5/02A23F5/204A23L5/00A23L7/00A23L7/104A23L21/00A23L21/25A23L23/00A23L31/00A23L33/105A23L11/50C12N1/14
Inventor KELLY, BROOKS JOHNLANGAN, JAMES PATRICK
Owner MYCOTECH
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