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Method for Promoting Respiratory Burst Activity Promoter and Immunostimulating in Type 2 Diabetic Patients

a promoter and respiratory burst technology, applied in immunological disorders, medical preparations, metabolism disorders, etc., can solve the problems of increasing the risk of infectious disease promote respiratory burst activity, and promote good ros” (respiratory burst)

Inactive Publication Date: 2016-06-30
OSATO INT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention provides a product that can boost the respiratory burst activity of patients with type 2 diabetes without affecting their blood glucose level. This is helpful for these patients as it promotes the presence of "good ROS" (respiratory burst) while reducing the presence of "bad ROS" (oxidative stress). This results in an increase in the immunity of these patients.

Problems solved by technology

The human peripheral blood mononuclear cells (PBMC) of patients with type 2 diabetes (T2D) result in a reduction in the respiratory burst activity, which increases the risk of infectious disease.

Method used

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  • Method for Promoting Respiratory Burst Activity Promoter and Immunostimulating in Type 2 Diabetic Patients
  • Method for Promoting Respiratory Burst Activity Promoter and Immunostimulating in Type 2 Diabetic Patients
  • Method for Promoting Respiratory Burst Activity Promoter and Immunostimulating in Type 2 Diabetic Patients

Examples

Experimental program
Comparison scheme
Effect test

example 1

Safety of FPP in T2D Patient

[0049]Before examining the in vivo effect of FPP in T2D patients, the safety of FPP in T2D patients was first confirmed.

[0050]In 17 patients diagnosed as T2D, blood before the start of FPP ingestion (base line (BL)) was collected in OSU Diabetic Clinic, and FPP (9 g / day) was ingested for subsequent 6 weeks, during which they came 2 times to the clinic. The test was completed by 2 times visiting to the clinic during subsequent wash-out. With every visiting to the clinic, respiratory burst ROS production, fasting blood glucose, lipid value, glycated hemoglobin (HbA1c), and lipid / protein peroxidation reaction were measured. Peripheral blood was collected from T2D patients in OSU Diabetic Clinic (see Table 1). For T2D patients, the period of 6-week FPP (9 g / day) ingestion and 2-week wash-out was provided.

TABLE 1Patient StatisticsTotal Number of Recruitments (n)22Number of Completed Study (n)17Age (Years)56.4 ± 12 Gender-Males12Gender-Females10Ethnicity-White6...

example 2

Improvement of Respiratory Burst Activity in Peripheral-Blood Mononuclear Cell of T2D Patient by FPP

[0054]The present inventors previously reported that the ex vivo treatment of human peripheral-blood mononuclear cells (PBMC) with FPP improved a reduction in respiratory burst due to T2D when stimulated with phorbol 12-myristate 13-acetate (PMA) (Non Patent Literature 4).

[0055]To verify whether or not the in vivo oral ingestion of FPP could improve respiratory burst activity in monocytes, peripheral-blood mononuclear cells (PBMC) were collected from T2D patients. PBMC were collected from T2D subjects during base-line, 2 and 6 weeks after starting the ingestion of FPP, and 2 weeks after wash-out. After stimulation with PMA (1 μg / ml) for 30 minutes, the production of reactive oxygen species (ROS) superoxide anion during respiratory burst in PBMC was measured by detection by chemiluminescence. Protein carbonyl and 4-hydroxynonenal (HNE-4) were measured by ELISA, as described above. The ...

example 3

Promotion of Intracellular Production of ATP and NADPH by FPP

[0060]The hyperphosphorylation of oxidase subunit p47 (PHOX) occurs when NADPH oxidase is active in intact cells. The present inventors previously reported that p47 (PHOX) was upregulated in PBMC ex vivo given FPP.

[0061]As described in [Materials and Methods], the human monocytic cell line, THP-1 cells, was cultured in the glucose-free medium for 48 hours, and, after removing glucose from the cells, cultured for 24 hours in a medium containing a normal glucose level (NG), a high glucose level (HG), FPP, or NG+FPP. Thereafter, the cells for the substance groups were measured for intracellular ATP, NADP, and NADPH by ELISA.

[0062]The results are shown in FIG. 3. ATP is necessary for phosphorylation reaction. In the human monocytic cell line, THP-1 cells, the ATP level was 75% higher for the glucose medium containing FPP (NG+FPP) than that for only the glucose medium (NG), suggesting that FPP promoted ATP production in the mon...

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Abstract

To confirm the safety of a fermented papaya preparation in type 2 diabetic patients while determining its in vivo effect on respiratory burst in human peripheral-blood mononuclear cells to find further health advantages for the type 2 diabetic patients. A respiratory burst activity promoter for improving the production of reactive oxygen species (ROS) induced by NADPH oxidase and promoting respiratory burst activity in type 2 diabetic patients, comprising a fermented papaya preparation as an active ingredient.

Description

TECHNICAL FIELD[0001]The present invention relates to a respiratory burst activity promoter for promoting respiratory burst activity, and an immunostimulant. Specifically, the invention relates to a respiratory burst activity promoter for improving the production of reactive oxygen species (ROS) induced by NADPH oxidase and promoting respiratory burst activity in type 2 diabetic patients, comprising a fermented papaya preparation (FPP) as an active ingredient, and an immunostimulant, based on improving the production of reactive oxygen species (ROS) induced by NADPH oxidase and promoting respiratory burst activity in type 2 diabetic patients, comprising FPP as an active ingredient.BACKGROUND ART[0002]FPP produced by fermenting the unripe fruit of Carica papaya Linn together with sugar using edible yeast contains increased maltose and maltotriose in a mixture with saliva compared to that in a mixture with water.[0003]The oral ingestion of FPP is expected to serve to improve the intes...

Claims

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Application Information

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IPC IPC(8): A61K36/185
CPCA61K2236/19A61K36/185A61P3/10A61P37/04A61P43/00A61K36/06
Inventor HAYASHI, YUKIYASUSEN, CHANDAN K.
Owner OSATO INT
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