Nanosystems as selective vehicles
a technology of nanosystems and selective vehicles, applied in the field of medical devices, can solve problems such as complicated monitoring and treatment of nanosystems
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example 1
on of the Nanoemulsions of the Invention
[0104]The preparation of the nanoparticles was carried out by means of the ethanol injection technique, for which a stock was prepared with the oil: surfactant ratio (sphingolipids in combination or not with other surfactants), necessary in each case (Organic phase, FO), and injected 100 μL (using a syringe of 0.5 mL insulin Myjector, U-100 Insulin, Terumo) on 900 μL of H2O (aqueous phase, FA) contained in a small vial of 2 mL, and under magnetic stirring. The formation of oil-in-water (O / W) nanoemulsions occurred spontaneously under these conditions, presenting a spherical morphology and giving places to homogenous populations (FIG. 1). The structure consists of an oil core stabilized by sphingomyelin and sometimes other lipids, and are in principle suitable systems for the encapsulation in the nucleus of hydrophobic molecules, being able to associate also another type of amphiphilic or hydrophilic molecules, which will be arranged preferably...
example 2
ion of HPLC Methods
[0106]High performance liquid chromatography (HPLC) was used to determine the association to the developed nanostructures, both of the associated ligands, and of the therapeutic molecules of interest.
[0107]For the optimization of the methods, the characteristics of each molecule were taken into account. As for the ligands, the compounds described in Table 2 are contemplated, which after their incorporation into the formulation, give rise to functionalized nanoemulsions as those shown in Table 3. In the case of the peptides, the analysis was carried out at 220 nm, with a combination of water and acetonitrile as the mobile phase, usually less than 60% acetonitrile being sufficient for elution. It was also important to add in the mobile phase a small percentage of TFA (0.2%), an ion-pairing agent, highly used at its high volatility (Agilent, 2013), which allows the separation of ionic substances on phase HPLC columns. reverse by controlling retention and selectivity....
example 3
lization of the Nanosystems
[0110]The developed nanosystems were functionalized with the ligands mentioned in table 3. The characterization of said developed nanosystems was performed in terms of size, dispersion, and surface charge, in the same way as in the case of nanoemulsions without functionalization (example 1). Next, the procedure followed is grouped by the type of preparation in four cases.
3.1. Functionalization with Lipid Conjugates of Peptides, LAPI, UROG y RPM (V:SM:LAPI 1:0,1:0,01, O:SM:UROG 1:0,2:0,01, y V:SM:RPM 1:0,1:0,05)
[0111]The association of this ligand to nanoemulsions was made by adding 50 μL of a stock of each of the derivatives after dissolution in ethanol (at a concentration of 1 mg / ml in the case of LAPI and UROG, or 0.5 mg / ml for RPM, to the organic phase, which was then injected with 1 ml of water under stirring, to obtain the functionalized nanoemulsions.
3.2. Functionalization with the Hydrophilic Peptides (Cationic Peptide Derived from Uroguaniline O:SM...
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