Cell potential measurement apparatus having a plurality of microelectrodes

a cell potential and measurement apparatus technology, applied in tissue culture, enzymology, after-treatment of biomass, etc., can solve the problems of damage to cells, difficult multi-point simultaneous measurement, and difficult measurement over a long period of tim

Inactive Publication Date: 2008-04-01
PANASONIC CORP
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Benefits of technology

[0017]It is preferable that a computer is provided to output the stimulation signal via a D/A converter, and at the same time, to receive and process an output signal arising from electric physiological activities of the cells via an A/D converter. As a result, the stimulation signal can be determined as an optional waveform on the screen or

Problems solved by technology

However, measurement by the insertion of these electrodes has the possibility of damaging the cells, and measurement over a long period of time is difficult to

Method used

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  • Cell potential measurement apparatus having a plurality of microelectrodes
  • Cell potential measurement apparatus having a plurality of microelectrodes
  • Cell potential measurement apparatus having a plurality of microelectrodes

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Embodiment Construction

[0030]This invention will now be described in detail by referring to the attached figures and the following examples. The examples are illustrative and should not be construed as limiting the invention in any way.

[0031]First, an integrated cell holding instrument used for a cell potential measurement apparatus of this invention will be explained. The integrated cell holding instrument 1, as shown as a perspective view in FIG. 1 and as an assembly diagram in FIG. 2, comprises a planar electrode 2, which is disposed with a plurality of microelectrodes and their drawer patterns on the surface of a glass plate, half-split holders 3, 4 for fixing the planar electrode 2 by holding it from the top and bottom, and a printed circuit board 5 on which these holders are fixed.

[0032]The planar electrode 2 is approximately the same as that disclosed in Laid-open Japanese patent application No. (Tokkai Hei) 6-78889 and others. The planar electrode 2 comprises, for example, a substrate made of a tr...

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Abstract

A cell potential measurement apparatus, which uses a planar electrode enabling a multi-point simultaneous measurement of potential change arising from cell activities, is provided which can conduct measurements accurately and efficiently as well as can improve convenience of arranging measurement results. According to the configuration of the cell potential measurement apparatus of this invention, it includes an integrated cell holding instrument, which includes a planar electrode provided with a plurality of microelectrodes arranged in a matrix form on the surface of a substrate, a cell holding part for placing cells thereon, drawer patterns from the microelectrodes, and electric contact points for outside connections; an optical observation means for optical observations of cells; a stimulation signal supply means to be connected to the cell holding instrument for providing electric stimulation to the cells; and a signal processing means to be connected to the cell holding instrument for processing an output signal arising from electric physiological activities of the cells. It is preferable that a cell culturing means is also provided for maintaining a culture atmosphere of the cells placed on the integrated cell holding instrument.

Description

[0001]Application 09 / 688,077, filed Oct. 13, 2000 and co-pending Application 09 / 169,188, filed Oct. 8, 1988, now RE 38,323 are each reissues of U.S. Pat. No. 5,563,067 (Application No. 08 / 464,116), filed Jun. 5, 1995.<?insert-end id="INS-S-00001" ?>FIELD OF THE INVENTION[0002]This invention relates to a cell potential measurement apparatus which is used in the field of electrical neurophysiology for measuring potential change associated with activities of nerve cells or nerve organs.BACKGROUND OF THE INVENTION[0003]Recently, medical investigations into nerve cells and the possibility of using nerve cells as electric elements have been actively pursued. When nerve cells are active, action potential is generated. This action potential arises from a change in ion concentration inside and outside the cell membrane which is accompanied by a change in ion permeability in nerve cells and thus from the change in cell membrane potential accompanied thereby. Therefore, measuring this po...

Claims

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Application Information

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IPC IPC(8): C12M1/34G01N33/487
CPCC12M41/46G01N33/48728
Inventor SUGIHARA, HIROKAZUKAMEI, AKIHITOKOBAYASHI, YASUSHITAKETANI, MAKOTOMITSUMATA, TADAYASU
Owner PANASONIC CORP
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