The invention relates to a method for measuring galactose content using enzyme colorimetry and enzyme-linked method technology, the composition and components of reagents, and the technical principle of its measurement is based on the development of galactose kinase, urea carboxylase, and hydrogen cyanide synthase. The series of catalytic reactions are completed, and the invention also relates to a galactose diagnosis / measurement kit. The assay method of the present invention has high sensitivity and small error, so the assay method and kit of the present invention can be widely used in clinical medicine / food inspection.