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Glycine Determination Method and Glycine Determination Kit

A determination method, glycine technology, applied in the direction of microbial determination/inspection, biological testing, biochemical equipment and methods, etc., can solve the problem of not being allowed to use

Inactive Publication Date: 2011-12-28
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] According to my country's "Hygienic Standards for the Use of Food Additives" GB2760, as a food additive, glycine is only allowed to be used in flavoring agents and soy milk, and the maximum limit is 1 gram per kilogram, but it is not allowed to be used in milk-containing beverages

Method used

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  • Glycine Determination Method and Glycine Determination Kit
  • Glycine Determination Method and Glycine Determination Kit
  • Glycine Determination Method and Glycine Determination Kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] Embodiment 1: Determination of glycine content in milk

[0091] 1) Preparation of standard samples

[0092] Dissolve a certain amount of glycine in water or buffer, and then adjust the concentration of glycine to 1 mmol / L;

[0093] 2) Pretreatment of samples to be tested

[0094] The milk sample is used as the sample to be tested without pretreatment;

[0095] 3) Blank sample

[0096] Described water or buffer solution are used as blank sample, and its glycine concentration is 0 mmol / L;

[0097] B. Preparation of reagent solution:

[0098] The glycine determination reagent of the present embodiment is a single-dose reagent, which contains:

[0099] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0100] Glycerin 1mol / L

[0101] NADP + 1.25mmol / L

[0102] Hydrogen cyanide synthase 16000U / L.

[0103] All these reagents were dissolved in deionized water according to the above concentrations and prepared for use.

...

Embodiment 2

[0115] Embodiment 2: the mensuration of glycine content in the milk

[0116] 1) Preparation of standard samples

[0117] Dissolve a certain amount of glycine in water, then adjust the concentration of glycine to 1 mmol / L, as a standard sample;

[0118] 2) Preparation of samples to be tested

[0119] The milk sample is used as the sample to be tested and does not need to be processed;

[0120] 3) Blank sample

[0121] Described water is used as blank sample, and its glycine concentration is 0 mmol / L;

[0122] B. Preparation of reagent solution:

[0123] Glycine Assay Reagent is a two-part reagent containing:

[0124] Reagent 1 with the following composition:

[0125] Phosphate buffer 100mmol / L

[0126] NAD + 2.25mmol / L;

[0127] Reagent 2 with the following composition:

[0128] Phosphate buffer 100mmol / L

[0129]Ethylene glycol 5mol / L

[0130] Hydrogen cyanide synthase 26000U / L.

[0131] All these reagents were dissolved in deio...

Embodiment 3

[0147] Embodiment 3: stability test

[0148] The operation mode of this embodiment is the same as that of Example 1, except that after the implementation of Example 1, the reagents used in Example 1 were stored in a sealed reagent bottle at 2-8° C. for half a year and one year.

[0149] Using the same standard sample, using the same new reagent as in Example 2 and the above-mentioned reagents stored for half a year and one year have been measured respectively, and other conditions are all the same as in Example 2, and the measurement results are as follows:

[0150] Table 1

[0151] Glycine content analysis error new reagent 1.03mmol / L ±0.001mmol / L Reagents stored for half a year 1.02mmol / L ±0.001mmol / L Reagents stored for one year 1.00mmol / L ±0.001mmol / L

[0152] The results in Table 1 show that using the kit of the present invention and the assay method of the present invention can ensure stable results, and the stability is at...

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Abstract

The invention relates to a method for measuring glycine content using enzyme colorimetry technology, the composition and ingredients of reagents, and the technical principle of the determination is based on the completion of the catalytic reaction of hydrogen cyanide synthase. The invention also relates to a glycine determination kit. The determination method of the invention has high sensitivity and small error, so the determination method and the kit of the invention can be widely used in food inspection.

Description

【Technical field】 [0001] The invention relates to the technical field of food inspection and determination, more specifically, the invention relates to a glycine determination method and a kit thereof. 【Background technique】 [0002] Glycine is a non-essential amino acid. Glycine has a unique sweet taste, which can ease the acid and alkali taste, cover the bitter taste of saccharin added in food and enhance the sweet taste. If the human body consumes too much glycine, not only will it not be absorbed and utilized by the human body, but it will also break the body's absorption balance of amino acids and affect the absorption of other amino acids, resulting in nutritional imbalance and affecting health. Glycine cannot be used by the body, it can only be decomposed and converted into heat energy, which will increase the burden on the liver. The nitrogen-containing compounds in the decomposition products have to be excreted through the urine, which will increase the burden on ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N21/31G01N35/00C12Q1/26
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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