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Glycine Determination Method and Glycine Determination Kit-9088

A technology of glycine and reagents, which is applied in the field of glycine determination methods and kits, and can solve the problems of not being allowed to use

Inactive Publication Date: 2011-12-28
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] According to my country's "Hygienic Standards for the Use of Food Additives" GB2760, as a food additive, glycine is only allowed to be used in flavoring agents and soy milk, and the maximum limit is 1 gram per kilogram, but it is not allowed to be used in milk-containing beverages

Method used

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  • Glycine Determination Method and Glycine Determination Kit-9088
  • Glycine Determination Method and Glycine Determination Kit-9088
  • Glycine Determination Method and Glycine Determination Kit-9088

Examples

Experimental program
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Embodiment l

[0126] Example 1: Determination of Glycine Content in Milk

[0127] 1) Preparation of standard samples

[0128] Dissolve a certain amount of glycine in water or buffer, and then adjust the glycine concentration to 1 mmol / L;

[0129] 2) Pretreatment of the sample to be tested

[0130] The milk sample is used as the sample to be tested without pretreatment;

[0131] 3) Blank sample

[0132] The water or buffer is used as a blank sample, and its glycine concentration is 0 mmol / L;

[0133] B. Preparation of reagent solution:

[0134] The glycine determination reagent in this embodiment is a single-dose reagent, which contains:

[0135] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0136] Glycerin 1mol / L

[0137] NADP + 0.75mmol / L

[0138] Hydrogen cyanide synthase 16000U / L

[0139] Ketoglutarate synthase 18000U / L

[0140] Pyruvate dehydrogenase 12000U / L

[0141] Reduced ferredoxin 5mmol / L

[0142] Succinyl-CoA 3mmol / L

[0143] Pyruvate 14mmol / L.

[...

Embodiment 2

[0156] Example 2: Determination of Glycine Content in Milk

[0157] 1) Preparation of standard samples

[0158] Dissolve a certain amount of glycine in water, and then adjust the glycine concentration to 1 mmol / L as a standard sample;

[0159] 2) Preparation of samples to be tested

[0160] Milk samples are used as samples to be tested and do not need to be processed;

[0161] 3) Blank sample

[0162] The water is used as a blank sample, and its glycine concentration is 0 millimoles / liter;

[0163] B. Preparation of reagent solution:

[0164] The reagent for determination of glycine is a two-dose reagent, which contains:

[0165] Reagent 1 consisting of:

[0166] Phosphate buffer 100mmol / L

[0167] NAD + 1.25mmol / L

[0168] Reduced ferredoxin 5mmol / L

[0169] Succinyl-CoA 5mmol / L

[0170] Pyruvate 15mmol / L;

[0171] Reagent 2 consisting of:

[0172] Phosphate buffer 100mmol / L

[0173] Ethylene glycol 5mol / L

[0174] Hydrogen cyanide synthase 16000U / L

[0175] Ketoglutarate synth...

Embodiment 3

[0193] Example 3: Determination of Glycine Content in Milk Samples

[0194] The operation mode of this embodiment is the same as that of embodiment 2, except that this embodiment:

[0195] B. Preparation of reagent solution:

[0196] Glycine determination reagent is a three-agent reagent, which contains:

[0197] Reagent 1 consisting of:

[0198] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0199] NAD + 1.25mmol / L

[0200] Reduced ferredoxin 6mmol / L

[0201] Succinyl-CoA 5mmol / L

[0202] Pyruvate 14mmol / L;

[0203] Reagent 2 consisting of:

[0204] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0205] Ammonium sulfate 1mol / L

[0206] Ketoglutarate synthase 28000U / L

[0207] Pyruvate dehydrogenase 50000U / L;

[0208] Reagent 3 consisting of:

[0209] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0210] Ammonium sulfate 2mol / L

[0211] Hydrogen cyanide synthase 26000U / L.

[0212] Add these reagents to ...

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Abstract

The invention relates to a method for measuring glycine content using enzyme cycle amplification method, enzyme colorimetric method and enzyme-linked method technology, the composition and components of reagents, the technical principle of which is based on the synthesis of hydrogen cyanide synthase and ketoglutarate The series of catalyzed reactions of the enzyme and pyruvate dehydrogenase are completed, and the invention also relates to a glycine determination kit. The determination method of the invention has high sensitivity and small error, so the determination method and the kit of the invention can be widely used in food inspection.

Description

【Technical Field】 [0001] The present invention relates to the technical field of food inspection and determination. More specifically, the present invention relates to a glycine determination method and a kit thereof. 【Background technique】 [0002] Glycine is a non-essential amino acid. Glycine has a unique sweet taste, can alleviate the acid and alkali taste, mask the bitter taste of added saccharin in food and enhance the sweetness. If the human body consumes too much glycine, not only can it not be absorbed and utilized by the body, but it will also break the body's absorption balance of amino acids and affect the absorption of other amino acids, leading to nutritional imbalance and affecting health. Glycine cannot be used by the human body and can only be decomposed and converted into heat energy, which will increase the burden on the liver. The nitrogenous compounds in the decomposition products will be excreted through urine, which will increase the burden on the kidneys...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N21/31G01N21/33C12Q1/32C12Q1/26
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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