The utility model discloses a high-strength anti-loosening bolt which comprises a bolt head, a bolt rod, a first rotating groove, a nut, a first threaded hole, a clamping groove, an anti-loosening structure, a rotating rod, a rotating head, a second rotating groove, a pointed part, a second threaded hole and a positioning rod. A first rotating groove is formed in the upper end face of the bolt head, a nut is in threaded connection with the outer wall of the bolt rod, first threaded holes are symmetrically formed in the two sides of the interior of the nut and are transversely formed, clamping grooves are symmetrically formed in the outer side wall of the bolt rod, and the clamping grooves communicate with the first threaded holes. The first threaded holes are internally provided with anti-loosening structures, each anti-loosening structure comprises a rotating rod, a rotating head, a second rotating groove and a pointed part, the locking strength of the nut can be improved, then the anti-loosening effect of the bolt is improved, and the bolt is not prone to falling off in the long-time using process.