Formulation comprising thymol useful in treatment of drug resistant bacterial infections
A bacterial infection, thymol technology, applied in antibacterial drugs, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve problems such as single multidrug tolerance of infection
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0057] The antibacterial activity of Trychyspermum ammi oil, thymol, and the thymol-free fraction of the oil was determined using a disk diffusion assay, expressed as the zone of growth inhibition produced on the lawn of resistant strains (Table 1).
[0058] large intestine rod
[0059] Here, CA8000 is a wild-type strain of E. coli, DH5α and ET8000 have a cryptic gyr mutation, that is, the gene encoding the Gyr A subunit of DNA gyrase responsible for DNA relaxation is modified. Since the DNA gyrase is altered, these mutants are resistant to quinolones. Specific mutations were confirmed by gene complementation by transfer of the plasmid that cloned gyr A. As shown in the table, the killing zone of thymol was greater in the case of gyr mutant strains tolerant to the above drugs. No activity was observed in the thymol-free fraction of the oil.
Embodiment 2
[0061] Sensitivity of nalidixic acid-tolerant Escherichia coli strains to different antibiotics determined by toxic agar
[0062] Independent mutants tolerant to nalidixic acid were isolated in E. coli by introduced and spontaneous gene mutations in the wild-type CA8000 strain. They were then tested for patterns of sensitivity to nalidixic acid and other next-generation quinolones and fluoroquinolones, since resistance to second- and third-generation drugs is also caused only by mutations in the gyrA gene.
[0063] Table 2 below shows the cross-resistance patterns of these mutants.
[0064] Escherichia coli
(Nal R ) strain
Growth on toxic agar medium containing
nalidixic acid
Oxolinic acid
Sifloxacin
50μg / ml
5μg / ml
5μg / ml
5μg / ml
5μg / ml
CAN 101
+
-
+
-
-
CAN 102
+
-
+
-
-
CAN...
Embodiment 3
[0068] Thymol against E. coli Nal R Biological evaluation of mutant activity
[0069] Test the sensitivity of the above-mentioned mutants to thymol, and further confirm the above-mentioned hypothesis, that is, Nal of Escherichia coli R The mutants were instead effectively killed by thymol, thus illustrating its use as a plant antibiotic to control resistant bacteria. Thymol was clearly shown in these assays against all Nal R Activity of the mutants, whether single or multidrug resistant (Table 3).
[0070] Mutations in E. coli
Body (Nal R ) strain
Thymol (mm of zone of inhibition)
50μg / plate
100μg / plate
250μg / plate
CAN 101
Tr.
1.5
4.0
CAN 102
Tr.
2.0
7.0
CAN 103
Tr.
2.0
4.0
CAN 104
Tr.
2.0
6.0
CAN 105
Tr.
2.0
5.0
CAN 106
1
2.0
4.0
CAN 107
1
3.0
5.0
CAN 108
...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More