Programmed freezing preservation method of kelp clone

Abstract

The invention discloses a procedure cooling storing method for the clone of sea tangle, which is characterized by the following steps: pretreating the material, balancing, cooling, resuscitating and removing the preservative. The invention improves the shortcomings of the preexisting subculture store clone technique and provides a cooing storing method for the clone of sea tangle, which can store the sea tangle in the ultra-low temperature with high survival rate and high degree of automatization and is facilitate for the standardization.

Description

1. Technical field [0001] The invention belongs to the technical field of marine biology, and relates to a large-scale economical algae-kelp germplasm preservation technology. 2. Background technology [0002] Kelp (Laminaria japonica Aresch) is an important economic algae in China. The life history of kelp is divided into two distinct generational stages: the sporophyte generation and the gametophyte generation. The gametophyte generation is the haploid stage of Laminaria japonica. ) and male gametophyte (male gametophyte) are isolated and cultured individually to form a clone. The traditional germplasm preservation technology is to subculture and preserve the clones. The traditional preservation technology has obvious deficiencies: (1) liquid culture is the only preservation method, and the method is single, which limits the scale and efficiency of preservation; (2) liquid culture Preservation generally needs to update the culture medium within half a month, the workload ...

AI Technical Summary

Problems solved by technology

The traditional germplasm preservation technology is to subculture and preserve the clones. The traditional preservation technology has obvious deficiencies: (1) liquid culture is the only preservation method, and the method is single, which limits the scale and efficiency of preservation; (2) liquid culture Preservation generally needs to update the culture medium within half a month, the workload is heavy, the operation is cumbersome, and frequent operations also increase the chance of germplasm contamination and mixing; (3) the kelp species cannot be aseptically treated before the spores are collected, and the entire preservation process is difficult. Under the condition of bacteria, the pollution of microorganisms, especially algae, cannot be completely eliminated; (4) Abnormal cell morphology and parthenogenesis occur from time to time during culture, and the culture factors need to be further optimized

The method of ultra-low temperature cryopreservation of algae is mainly two-step freezing method (two-step freezing). In this method, an antifreeze protective agent is added to the preservation material first, and pre-freezing is performed, and then the pre-frozen two-step method is frozen in macroeconomic algae. The species used are laver and wakame, and there is still a blank about the two-step cryopreservation of kelp clones