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Northern aweto strain and its selection breeding method

A technology for Cordyceps militaris and Cordyceps militaris seeds is applied in the field of breeding and producing high-yield and high-quality strains of Cordyceps militaris, which can solve the problems of variation and loss of excellent traits of strains, and achieve the effect of avoiding the accumulation of homokaryotes.

Inactive Publication Date: 2008-07-16
SHANGHAI ELITE AGRI SCI TECH GROUP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to solve the problem that the prior art cannot effectively solve the problem of heterokaryon decomposition and homokaryon accumulation in the Cordyceps militaris strain, which leads to the degradation of the strain and the lack of fruiting bodies and the variation and loss of excellent traits of the strain during the cultivation process. A method for effectively screening and optimizing the excellent traits of strains, and using them in production practice to produce stable, reliable, high-yielding, high-quality strains of Cordyceps militaris

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The surface of Cordyceps militaris sporocarps collected in the field or artificially cultivated with mature asthecia is sterilized, placed on top of a sterile PDA culture plate, and cultured in a culture room with a temperature of about 20-25°C until ascospores When ejected to the surface of the PDA medium and germinate and grow into tiny colonies visible to the naked eye, single-spore colonies are picked and transferred to new PDA culture plates or test tubes for cultivation; this process can be assisted by microscope observation to observe ascospore germination The process, and ensure that the picked ascospore monospore colonies.

[0024] After the obtained ascospore monospore strains were purely cultured and multiplied on the PDA medium, samples were taken to observe the characteristics of the anamorphic sporulation structure of each bacterial strain under a microscope, and the anamorphic sporulation structure was respectively cephalosporin type and Paecilomyces type...

Embodiment 2

[0027] After the fruiting bodies of Cordyceps militaris with mature asthecia collected in the field or cultivated artificially are surface-sterilized, they are placed on top of a sterile culture plate poured with PDA-modified medium, and placed in a culture room with a temperature of about 20-25°C. Medium culture, when the ascospores are projected onto the surface of the PDA-improved medium and germinate and grow into tiny colonies visible to the naked eye, pick the single-spore colonies and transfer them to a new culture plate or test tube of the PDA-improved medium for future use; this process Observation can be assisted by a microscope to observe the process of ascospore germination, and to ensure that the ascospore monospore colony is picked. The PDA modified medium formula used was: 200 g potato, 20 g sucrose, 3 g peptone, 3 g potassium dihydrogen phosphate, 1.5 g magnesium sulfate, 10 mg vitamin B 1 , 1000 ml of water. Add another 20 grams of agar to the solid medium. ...

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Abstract

The invention discloses a breeding making method of north Chinese caterpillar fungus, which comprises the following steps: separating ascospore monospore from north Chinese caterpillar fungus; proceeding pure culture; obtaining monospore strain of ascospore; marking number for structure; allocating the fruiting body of north Chinese caterpillar fungus; detecting the production and quality of fruiting body; breeding; blending according to proportion; obtaining the product; avoiding karyosome from accumulating in the culture course.

Description

technical field [0001] The invention relates to the technical field of artificial cultivation of Cordyceps militaris, in particular to a method for breeding and producing high-yield and high-quality strains of Cordyceps militaris. Background technique [0002] Cordyceps militaris, also known as Cordyceps militaris, belongs to Ascomycotina, Sclerotinia, Ergotaceae, Ergotaceae, Cordyceps genus in fungal taxonomy, and is the same as Cordyceps sinensis. kind. Cordyceps fungi are a general term for a class of insect fungus complexes that are parasitic on the underground fruiting bodies of insects, spiders or plants of the genus Macrocystis. Cordyceps militaris is the model species of the fungus of the genus Cordyceps. In the wild, it mainly hosts the larvae and pupae of some insects of the order Lepidoptera, Coleoptera and Diptera. Traditional Chinese medicine records its efficacy as "sweet in taste, flat in nature, beneficial to the lungs and kidneys." , replenish the essence,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14
Inventor 高新华吴畏沈明陈伟张红霞
Owner SHANGHAI ELITE AGRI SCI TECH GROUP