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Process for preparing self-fibroblast by skin prefibroblast

A technology of fibroblasts and precursor cells, applied in artificial cell constructs, biochemical equipment and methods, animal cells, etc., can solve the problems of high long-term recurrence rate, side effects that do not conform to human physiology, etc.

Inactive Publication Date: 2008-09-03
毛曦
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned treatment methods have the disadvantages of high long-term recurrence rate, certain side effects and inconformity with human physiology.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Method of separating and purifying skin fibroblast precursor cells by cell engineering technology to prepare autologous fibroblasts: from 9mm 3 Fibroblast precursor cells were isolated from a small piece of skin tissue. After purification, the fibroblast precursor cells were cultured to differentiate into fibroblasts and proliferate to 4×10 7 One, the prepared cells are mature fibroblasts with a passage number of no more than 5 times and in an active stage of function, and the method for preparing the cells consists of the following steps:

[0025] (1) Under sterile conditions, obtain a 9mm 3 Small pieces of skin tissue;

[0026] (2) Isolate fibroblast precursor cells from skin tissue by mechanical dissociation cell method, and carry out transplantation culture, the steps are as follows: 1) First, put the trimmed and washed tissue block into a small beaker, and use ophthalmology Cut and cut the tissue repeatedly until it looks like a paste; 2) Add 3ml of culture mediu...

Embodiment 2

[0034] Method of separating and purifying skin fibroblast precursor cells by cell engineering technology to prepare autologous fibroblasts: from 15mm 3 Fibroblast precursor cells were isolated from a small piece of skin tissue. After purification, the fibroblast precursor cells were cultured to differentiate into fibroblasts and proliferate to 6×10 7 One, the prepared cells are mature fibroblasts with no more than 4 passage times and are in the active stage of function, and the method for preparing the cells consists of the following steps:

[0035] (1) Under sterile conditions, obtain a 18mm 3 Small pieces of skin tissue;

[0036] (2) Isolate fibroblast precursor cells from skin tissue by mechanical dissociation cell method, and carry out transplantation culture, the steps are as follows: 1) First, put the trimmed and washed tissue block into a small beaker, and use ophthalmology Cut and cut the tissue repeatedly until it looks like a paste; 2) Add 3ml of culture medium int...

Embodiment 3

[0044] Method of separating and purifying skin fibroblast precursor cells by cell engineering technology to prepare autologous fibroblasts: from 21mm 3 Fibroblast precursor cells were isolated from a small piece of skin tissue. After purification, the fibroblast precursor cells were cultured to differentiate into fibroblasts and proliferate to 8×10 7 One, the prepared cells are mature fibroblasts with no more than 3 passage times and are in the active stage of function, and the method for preparing the cells consists of the following steps:

[0045] (1) Under sterile conditions, obtain a 21mm 3 Small pieces of skin tissue;

[0046] (2) Isolate fibroblast precursor cells from skin tissue by mechanical dissociation cell method, and carry out transplantation culture, the steps are as follows: 1) First, put the trimmed and washed tissue block into a small beaker, and use ophthalmology Cut and cut the tissue repeatedly until it looks like a paste; 2) Add 3ml of culture medium int...

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Abstract

The invention relates to a method used skin fiber forming precursor cell to manufacture own body fiber forming cell. It produces fiber forming cell by application cell engineering technique. The formed fiber forming cell can be used in cell transplantation. And it belongs to biological cell engineering technique field. It uses cell engineering technique to separate and purify skin fiber forming precursor cell. And its manufacturing includes the following steps: separating fiber forming precursor cell from 9mm3-21mm3 skin tissue; culturing it to form fiber forming cell after purifying; breeding to 4*107-8*107. The formed cell transfer culture times are not over 5. The formed own body fiber forming cell can be used in cell transplantation of curing female incontinence and removing wrinkle and pitting scar. After it is transplanted to dermal layer, its reparation is nature physiological type, and not happen side effect of immunological rejection.

Description

technical field [0001] The invention relates to a method for preparing autologous fibroblasts by using skin fibroblast precursor cells and relates to a method for preparing fibroblasts by using cell engineering technology, and the produced fibroblasts can be used for cell transplantation. It belongs to the technical field of biological cell engineering. Background technique [0002] Fibroblasts are the major cellular component of loose connective tissue. Fibroblasts produce collagen fibers, elastic fibers, and reticular fibers, as well as matrix glycosaminoglycans and glycoproteins. During wound repair, fibroblasts become larger in size and have increased cytoplasmic basophilia. Although fibroblasts are differentiated cells, they also possess plasticity, which can change their morphological and functional characteristics to adapt to the specific needs of their microenvironment. During wound healing, local fibroblasts can transform into cells with the structural characteri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/08C12N5/071
Inventor 毛曦
Owner 毛曦
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