Method for preparing radioactive arsenic compound and usage for curing tumor
An arsenic compound and radioactive technology, applied in the direction of radioactive preparations, radioactive carriers, antineoplastic drugs, etc. in the body, can solve the problems not mentioned
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Embodiment 1
[0068] Embodiment 1. Preparation of radioactive arsenic trioxide
[0069] Put 5 mg of arsenic trioxide powder (available from Taiwan Dongyang Pharmaceutical Company) into a quartz tube (Quartz glass, Tosiba, Japan), and the mouth of the quartz tube was completely sealed with a gas / oxygen mixed flame. The quartz tube was wrapped with aluminum foil and put into the irradiation target of a special aluminum tube for irradiation, and the neutron irradiation treatment was carried out for 30 hours under the operating conditions that would make the formed product have a radioactivity of 2-4mCi.
[0070] The irradiated quartz tube was cut with a cutting tool, and 3ml of 1N NaOH solution was added to the cut quartz tube to dissolve As 2 o 3 . After 20 minutes, 1 ml of 1N HCl solution was added, and finally 1 ml of normal saline was added to adjust the solution concentration to 1 mg / ml. The obtained solution containing arsenic trioxide irradiated with neutrons was subjected to gamm...
Embodiment 2
[0076] Example 2. Establishment of an animal model with liver tumors
[0077] Operation method:
[0078] The tumor cells used to establish the animal model with liver tumors were rat liver tumor cells purchased from the American Type Culture Collection (ATCC), P.O. Box 1549, Manassas, VA 20108, USA] Strain N1-S1 (rathepatoma cell line N1-S1), through continuous subculture to establish master cell bank and working cell bank.
[0079] Rat liver tumor cells N1-S1 are suspension cells, which were suspended in 1% penicillin (GibcoBRL ) with 10% fetal bovine serum (GibcoBRL ) of Iscove's Modified Dulbecco's Medium (IMDM) (GibcoBRL ), and at 5% CO 2 cultured in a constant temperature incubator at 37°C.
[0080] In culture to 1.5 x 10 6 Cells / ml, take out 4×10 6 Cells / 0.06ml of N1-S1 cells were surgically implanted between the liver and liver viscera capsule of male rats (Spawn Dawley, 5 weeks old, 150 g), and the wound was sutured. After continuing to feed the rats for 10 ...
Embodiment 3
[0089] Example 3. Biodistribution test (Biodistribution test)
[0090] Hepatic artery injection:
[0091] The hepatic artery and liver tissue of the rat were surgically stripped, and a PE-10 tube (PE-10 tube) was inserted into the hepatic artery, and then injected with the neutron-irradiated arsenic trioxide prepared in Example 1. After the injection, the PE-10 tube was drawn out, and the blood vessel was sealed and fixed with surgical thread, and then the wound was sutured.
[0093]The test injection solution prepared in Example 1 containing arsenic trioxide treated with neutron irradiation was directly injected into the rat's tail vein with a syringe with a 25-gauge needle.
[0094] Operation method:
[0095] Rats suffering from liver tumors were injected with a certain amount of radioactive arsenic trioxide prepared in Example 1 through the hepatic artery and tail vein respectively (radioactive range is 2.77-3.25mCi / ml, concentration is 1mg / ...
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