Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for concentrating virus in sewage or sewage treatment plant tail water

A sewage treatment plant and sewage technology, applied in adsorption water/sewage treatment, water/sludge/sewage treatment, centrifugal separation water/sewage treatment, etc. and other problems, to achieve the effect of reducing virus death, large amount of treated water and low cost

Inactive Publication Date: 2009-07-08
TSINGHUA UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many concentration methods currently available, and each has its own strengths, the complex water quality conditions in the actual water body affect the effect of virus concentration. In order to be better applied to water quality safety management, further exploration is still needed
[0014] Compared with drinking water, the water quality conditions of urban domestic sewage and sewage plant tail water are complex, with high organic matter content, and are easy to be blocked by membrane filtration.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for concentrating virus in sewage or sewage treatment plant tail water
  • Method for concentrating virus in sewage or sewage treatment plant tail water
  • Method for concentrating virus in sewage or sewage treatment plant tail water

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1, establishment of virus concentration method in sewage or sewage treatment tail water

[0046] The invention utilizes the solid particle adsorption-elution method to establish a virus concentration method suitable for domestic sewage with complex water quality conditions and tail water of sewage plants. Concentration of sewage viruses by solid particle adsorption and elution basically includes steps such as pre-filtration, solid particle adsorption, elution, and concentration.

[0047] In this embodiment, domestic sewage and secondary effluent are used as experimental objects to optimize various conditions of the virus concentration method in sewage or sewage treatment tail water.

[0048] Measuring the turbidity and COD value of domestic sewage and secondary effluent (the effluent after secondary treatment of domestic sewage), the turbidity of the sewage used in this research is 10-50NTU, and the COD is 200-250mg / L; the turbidity of the secondary effluent ...

Embodiment 2

[0143] Example 2, Effect Identification of Virus Concentration Method in Tail Water of Sewage or Wastewater Treatment Plant

[0144] 1. Water sample collection method

[0145] Plastic sample bottle for sampling, minimize the contact time with air when taking water, hold the bottom of the sample bottle with your hand, quickly immerse the bottle under the water surface, then turn the bottle mouth to the direction of water flow, when the water sample is filled to 2 / 3 of the bottle volume, Plug the bottle cap in the water and remove the water surface. The retrieved water samples were stored in a refrigerator at 4°C, and the experimental operation was completed as soon as possible.

[0146] 2. Actual water sample collection location and sampling volume

[0147] The monitoring locations of actual water samples include: Beijing Q sewage treatment plant (influent, secondary effluent and reclaimed water), G sewage treatment plant (influent, secondary effluent and reclaimed water), X ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle size (mesh)aaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for concentrating viruses in sewage or tail water of sewage treatment plants. The method for concentrating the virus comprises the following steps: 1) adjusting water sample conditions: getting sewage or sewage treatment plant tail water samples, adding Al3+, making the final concentration of Al3+ be 0.5-1mol / L, and adjusting the pH value to 3.0-1mol / L 3.5; 2) Adsorption: add silica gel particles to the water sample in step 1), and stir for adsorption; 3) Elution: collect the silica gel adsorbed in step 2), wash it with pH 3.0-3.5H2SO4 solution, and put it into pH Vortex in the 9.0-9.5 urea-lysine buffer, centrifuge to collect the supernatant to obtain the eluate; 4) Concentrate the eluate: Concentrate the eluate obtained in step 3) by ultrafiltration. The method of the present invention not only has the advantages of relatively high recovery rate, large amount of treated water, stable effect, simple operation, low cost, etc., but also can directly use the concentrated sample for molecular biological operations, which is a rapid and accurate detection of the The virus provides strong technical support.

Description

technical field [0001] The invention relates to a method for concentrating viruses in sewage or tail water of sewage treatment plants. Background technique [0002] Pathogenic microorganisms transmitted through water are a major hidden danger to human health and ecological safety, and about 1 / 4 of the diseases in the world are directly or indirectly caused by pathogenic microorganisms in water (Gerba, C.P., Pathogens in the environment. In: Pepper, I.L. , Gerba, C.P., Brusseau, M.L. (Eds.), PollutionScience. Academic Press, New York, 1996. p.279-299). The pathogenic microorganisms in the water environment are mainly divided into three types: pathogenic bacteria, viruses and protozoa. Among them, the content of viruses in the water environment is low, but they are highly pathogenic and various. Viruses in the water environment mainly come from human and animal excrement. After these viruses enter the water environment, they remain highly infectious and pathogenic for a long ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C02F9/02C02F1/50C02F1/28C02F1/38C02F1/52
Inventor 何苗李丹施汉昌杨万胡昕
Owner TSINGHUA UNIV