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Method for producing succinic acid by using ferment of molasses raw material

A technology of succinic acid and molasses, applied in microorganism-based methods, biochemical equipment and methods, fermentation, etc., to achieve the effect of reducing the cost of culture medium and alleviating the shortage of petrochemical resources

Inactive Publication Date: 2009-12-02
HANGZHOU XINFU TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no industrial example of using molasses raw material to ferment succinic acid

Method used

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  • Method for producing succinic acid by using ferment of molasses raw material
  • Method for producing succinic acid by using ferment of molasses raw material
  • Method for producing succinic acid by using ferment of molasses raw material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Seed medium (per L): glucose 5g, yeast extract 5g, K 2 HPO 4 ·3H 2 O 1.0 g, NaH 2 PO 4 ·2H 2 O1.0g, mixed with vitamin 1ml, pH7.0. Anaerobic culture at 37°C for 24h.

[0033] Fermentation medium (per L): processed cane molasses (total reducing sugar) 65g, yeast extract 15g, corn steep liquor 20g, MgCl 2 0.2g, Na 2 HPO 4 ·12H 2 O 1.5g, NaH 2 PO 4 ·2H 2 O 1.5g, pH 6.5. Anaerobic incubation at 37°C for 48h.

[0034] Molasses is processed as follows:

[0035] First, the raw molasses (about 510 g / L reducing sugar) was diluted with tap water to a sugar concentration of 300 g / L (hereinafter referred to as the diluted molasses), and then processed by the following methods.

[0036] (1) Sulfuric acid treatment: The diluted molasses was adjusted to pH 3.5 with 1 mol / L sulfuric acid solution, kept at rest for 6 h, centrifuged, and the supernatant was taken and adjusted to pH 6.5 with 5 mol / L NaOH.

[0037] (2) Potassium ferrocyanide treatment: add 0.1 g of potassium ...

Embodiment 2

[0045] According to the method of Example 1, in the anaerobic flask fermentation experiment, the sugar cane molasses was treated with sulfuric acid, and the initial reducing sugar concentration was 65 g / L, and different inorganic and organic nitrogen sources were added to the fermentation medium (the total nitrogen content was basically similar), their contents are (per L): NH 4 Cl 5.3g, KNO 3 9.6g, peptone 12g, corn steep liquor (solid content ≥55%) 25g, yeast extract 15g and dry yeast powder 12g. The effects of various nitrogen sources on the production of succinic acid from fermented sugarcane molasses are shown in Table 2.

[0046] Table 2 Effects of adding different nitrogen sources on the production of succinic acid from fermented sugarcane molasses (fermented in anaerobic flasks)

[0047]

[0048] Comparing the above nitrogen sources, yeast extract is the best. When the concentration of yeast extract is 15g / L, the output of succinic acid reaches 49.7g / L, the yield...

Embodiment 3

[0050] According to the method of Example 1, in the anaerobic flask fermentation experiment, the sugarcane molasses was treated with sulfuric acid. When the initial reducing sugar concentrations were 35, 50, 65, 80, and 100 g / L, the results were shown in Table 3:

[0051] Table 3 Effects of different initial reducing sugar concentrations on the production of succinic acid from fermented sugarcane molasses (anaerobic flask fermentation)

[0052]

[0053] As can be seen from the above table, when the initial reducing sugar concentration is 65g / L, the output of succinic acid is the highest, reaching 50.6g / L, the yield of succinic acid to consumed sugar is 83.70%, the utilization rate of sugar is 93.0%, and the production strength is 1.05g. / L·h.

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Abstract

The invention discloses a method for producing succinic acid by fermenting molasses raw materials, which belongs to the technical field of biomanufacturing. The present invention uses the microorganism Actinobacillus succinogenes CGMCC1593 screened by the inventor from the rumen to utilize waste molasses from sugar production (containing The concentration of total reducing sugar is 50-100g / L) to carry out batch or fed-batch anaerobic fermentation for 30-60h to reach the concentration of 30-60g / L of succinic acid, and the yield of succinic acid to consumed sugar is 70%-84%. The strength is 0.9-1.2g / L·h, and the sugar utilization rate is 90%-95%. The outstanding advantage of the present invention is that succinic acid is produced by fermenting and producing succinic acid by using cheap molasses raw material instead of pure glucose as a carbon source, utilizing renewable raw materials, which can alleviate the shortage of petrochemical resources for chemically synthesizing succinic acid, and is environmentally friendly.

Description

technical field [0001] The invention relates to a method for producing succinic acid by using molasses raw material fermentation, and belongs to the technical field of biological manufacturing. Background technique [0002] Succinic acid (succinic acid), also known as succinic acid, the molecular formula is C 4 H 6 O 4 , with a molecular weight of 118.09, is a common natural organic acid that widely exists in humans, animals, plants and microorganisms. [0003] Succinic acid is an important four-carbon compound in industry. It is widely used in food, medicine, pesticides, dyes, spices, paints, plastics and materials industries as organic synthesis raw materials, intermediate products or specialized chemicals. Its largest market is for surfactants, detergents, green solvents, ion chelators, biodegradable plastics and other fields. It is used in the food industry as an acidulant, pH improver, flavor and antibacterial agent, and as a raw material or intermediate for the pro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/46C12R1/01
Inventor 过鑫富孙志浩郑璞刘宇鹏
Owner HANGZHOU XINFU TECH CO LTD
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