Method for establishing brazilian panama rubber tree suspending cell line

Abstract

The invention discloses a method for establishing a suspension cell line of Hevea brasiliensis, which comprises the preparation process of explants, the induction process of embryogenic callus, the subculture process of embryogenic callus, and the suspension culture process of embryogenic callus. The invention has simple technological process, low production cost, rapid and stable multiplication of the established suspension cell line, and can be subcultured for a long time, and solves the problem of conventional Hevea brasiliensis embryogenic callus being affected by seasons and climates, as well as large-scale multiplication and costing a lot The problem of manpower and material resources provides an important foundation for the large-scale commercialization, factory production and large-scale field planting of Hevea brasiliensis root clones, and for the creation of Hevea brasiliensis using biotechnology methods such as somatic cell hybridization, genetic transformation and mutant induction. The new excellent germplasm resources provide new approaches and conditions, laying the foundation for the research and development of Hevea brasiliensis bioreactors.

Description

technical field

[0001] The invention belongs to the technical field of plant cell engineering, and more specifically relates to a method for establishing a suspension cell line of Hevea brasiliensis. Background technique

[0002] Natural rubber is one of the four major industrial raw materials and an extremely important strategic material. At present, among more than 2,000 kinds of rubber-producing plants in nature, about 99% of commercial natural rubber comes from Hevea brasiliensis. Hevea brasiliensis is a perennial tree belonging to the family Euphorbiaceae, and the conventional breeding cycle is 20 to 30 years. The ultra-long breeding cycle has greatly restricted the selection and renewal of new varieties of Hevea brasiliensis. In order to broaden the breeding approaches and innovate breeding methods of Brasilia, since the 1950s, breeders from all over the world have begun to study the application of biotechnology in Brasilian breeding. The success of somatic embryogen...