Method for reusing stems and leaves of potato
A technology of potato stems and leaves, volatile oil, applied in chemical instruments and methods, production of fat, essential oils/spice, etc., can solve the problem of not being used, piling up on the field and letting them rot or discarded or burnt, waste of agricultural resources, etc. question
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Embodiment 1
[0006] Embodiment 1 (steam distillation method extraction potato stem and leaf volatile oil)
[0007] In a flask equipped with a steam distillation device, steam distillation is carried out after adding crushed dry potato stems and leaves. The effluent was extracted with ether, separated, the organic layer was dried with anhydrous sodium sulfate and then filtered to remove the desiccant. The filtrate was concentrated with a rotary evaporator to remove the ether solvent to obtain the volatile oil of potato stems and leaves with a special smell. The volatile oil was analyzed by GC-MS (GC analysis conditions: the initial temperature was 60°C, the temperature was raised to 100°C at a rate of 8°C / min, kept for 2min, and then the temperature was raised to 250°C at a rate of 8°C / min. The temperature of the injection port was 260°C, and the Gas (helium) flow rate 1mL / min. Split ratio 60:1. MS analysis conditions: ionization mode EI, electron energy 70eV, ion source temperature 260 ° C...
Embodiment 2
[0008] Embodiment 2 (determination of antioxidant performance)
[0009] The essential oil obtained in Example 1 was formulated into an ethanol solution with a concentration of 0.104 g / ml for the determination of eliminating DPPH free radicals.
[0010] Determination method: Add 2.5mL of DPPH absolute ethanol solution (concentration 75μmol / L) and a certain amount of volatile oil sample ethanol solution into a 10mL test tube, then add a certain amount of absolute ethanol to make the total volume 3mL, and place it at room temperature for 30min Afterwards, the absorbance value was measured at 517nm, and the clearance rate was calculated by the following formula:
[0011] S = A 0 - ( A - A b ) A 0 ...
Embodiment 3
[0016] Embodiment 3 (determination of elimination ability to nitrite)
[0017] Using the essential oil ethanol solution prepared in Example 1 (concentration: 0.104 g / mL), the ability of the essential oil to eliminate nitrite was determined.
[0018] Determination method: Add 5mL (concentration 0.5mol / L, pH=3.0) of sodium citrate-hydrochloric acid buffer to a 10mL volumetric flask, add 1mL of NaNO 2 Solution (100mg / L) and a certain amount of ethanol solution of the above-mentioned essential oils, dilute to the mark with ethanol, and react at 37°C for 1h. Pipette 1mL of reaction solution in 7cm 2 Add 2mL of 0.4% (mass fraction) p-aminobenzenesulfonic acid solution and 1mL of 0.2% (mass fraction) N-1-naphthylethylenediamine hydrochloride to a petri dish, shake well and place it for 15min, then use a spectrophotometer Measure the absorbance value at 540nm (A x ), and the clearance rate was calculated by the following formula.
[0019] Calculation method: clearance rate = (A 0...
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