A microcapsule of egg yolk immunoglobulin, preparing process and use thereof
A technology of immunoglobulin and microcapsules, which is applied in microcapsules, capsule delivery, diseases, etc., and can solve the problems of uneven size of microcapsules and low encapsulation rate
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[0138] 3. Preparation of microcapsules. Preparation of microcapsules can be accomplished in two ways:
[0139] The first preparation method:
[0140] Preparation of capsule material solution: dissolve the biodegradable capsule material in a solvent mixed with dichloromethane and acetonitrile at a ratio of 1:99 to 99:1 at a concentration of 3%-10%, and add 0.1%-5% Tween (Tween) series or Span (Span) series or Poloxamer (Poloxamer) series or a mixture of the three in any proportion as an emulsifier;
[0141] Preparation of IgY aqueous solution: Dissolve chicken immunoglobulin (IgY) in water according to the concentration of 10-100 mg / ml;
[0142] Primary emulsification: add the IgY aqueous solution to the capsule material solution, and emulsify by mechanical stirring and ultrasound to obtain the first mixed phase, which is the binary mixed phase-water-in-oil (O / W) phase;
[0143] Secondary emulsification: Add this binary mixed phase to the aqueous solution containing 1-10% PV...
specific Embodiment 1
[0159] The following is an example of the preparation of a microcapsule that uses the polymeric anhydride polymer PCL as the capsule material to wrap the anti-caries chicken yolk immunoglobulin (anti-caries IgY) and the application of the above-mentioned microcapsules for preparing anti-caries series products, further The technical solution of the present invention is described.
[0160] 1. Preparation of anti-caries chicken yolk immunoglobulin (anti-caries IgY)
[0161] The study found that the main cause of dental caries is Streptococcus mutans with serotype C, and at the same time, it was found that the anti-caries chicken yolk immunoglobulin (anti-caries IgY) prepared by using the Streptococcus mutans with C serotype as antigen not only It has inhibitory effect on C-type Streptococcus mutans and has different inhibitory effects on other serotypes of Streptococcus mutans, and this inhibitory effect can directly and effectively prevent and reduce the incidence of dental cari...
Embodiment 1
[0177] Preparation of capsule material solution: Dissolve PCL capsule material at a concentration of 10% in 75ml of solvent mixed with dichloromethane and acetonitrile in a ratio of 30:70 and add 5% Tween 20 as an emulsifier;
[0178] Preparation of anti-caries IgY aqueous solution: dissolve anti-caries IgY in 30ml of water at a concentration of 50mg / ml;
[0179] Primary emulsification: add the anti-caries IgY aqueous solution to the capsule material solution, stir at a stirring speed of 300 rpm for 30 minutes, and then emulsify with 100 watts of ultrasound for 1 minute to obtain the first mixed phase, which is the binary mixed phase- Oil-in-water (O / W) phase;
[0180] Secondary emulsification: Add the above-mentioned binary mixed phase into 500ml aqueous solution containing 8% PVA dispersant, first stir at 300rpm for 30 minutes, and then emulsify with 100 watts of ultrasound for 1 minute to form the second mixed phase That is, the ternary emulsion of water-in-oil-in-water (W...
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