Method for separating purifying ursolic acid from loquat leaf

A technology for separation and purification of ursolic acid, applied in medical preparations containing active ingredients, organic chemistry, pharmaceutical formulas, etc., can solve problems such as waste and unused applications, and achieve reduced preparation costs, improved dissolution rate, and low boiling point Effect

Inactive Publication Date: 2007-09-19
FUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In loquat producing areas, loquat leaves, flowers, and cores w

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Using loquat leaves produced in Putian, Fujian as raw materials, fresh loquat leaves are washed, dried (air-dried, dried or sun-dried), pulverized, and sieved with 20-30 meshes to obtain loquat leaf powder, and 2.6kg of loquat leaf powder is put into extraction. In the tank, use 85% methanol-acetone (1:1) aqueous solution of 12 times the dry weight (W / W) of loquat leaf powder to reflux and extract for 2 hours at 72°C, filter, and the filtrate is at -0.05~-0.09MPa. , under the condition of 50~70 ℃, the solvent is recovered under reduced pressure, vacuum concentrated into loquat leaf total triterpene acid extract; under 72 ℃ condition, the loquat leaf total triterpene acid extract uses 3 times the amount (W / W) of pH 90% methanol-acetone (1:1) aqueous solution (W / W) with a value of 10.9 was used to fully extract the total triterpene acid of loquat leaves, stand for aging, filter to remove insolubles, and add 1.3% (W / V) to the filtrate while it was still hot. Activated carb...

Embodiment 2

[0039] Put 2.8kg of loquat leaf powder degreased with petroleum ether of 20~30 meshes into the extraction tank, use 20 times the dry weight (W / W) of loquat leaf powder with a pH value of 3.2 70% methanol aqueous solution, under the condition of 50 ℃ Extracted twice, 3 hours each time, combined the extracts, filtered, the filtrate was recovered under reduced pressure under the conditions of -0.05~-0.09MPa and 50~70°C, and the solvent was recovered under reduced pressure, and concentrated in vacuo to obtain the loquat leaf total triterpene acid extract; 50 Under the condition of ℃, the total triterpene acid extract of loquat leaves was fully extracted with 15 times the amount (W / W) of 92% methanol aqueous solution (W / W) with a pH value of 8.0, and the total triterpenoid acids of loquat leaves were fully extracted, and then left to stand for aging and filtration. Remove insoluble matter, add 0.5% (W / V) activated carbon to the filtrate while hot for decolorization; add 50% sulfuric...

Embodiment 3

[0043] Put 2.9kg of 20-30 mesh loquat leaf powder that has undergone carbon dioxide supercritical extraction and deoiling treatment into the extraction tank, and use 5 times the dry weight (W / W) of the loquat leaf powder to extract at 80 °C. 2 hours, filter, under the conditions of -0.05~-0.09MPa and 50~70 ℃, the filtrate is recovered under reduced pressure, and the solvent is concentrated in vacuo to obtain the loquat leaf total triterpene acid extract; under the condition of 80 ℃, the loquat leaf total triterpene acid extract The extract was fully extracted with 10 times the amount (W / W) of 80% methanol aqueous solution (W / W) with a pH value of 8.9, and the total triterpene acid of loquat leaves was fully extracted. % (W / V) activated carbon for decolorization; the decolorized filtrate was added to a saturated aqueous citric acid solution while hot, and the final methanol concentration in the control system reached 25% (W / W) and the pH value was 5.5, and slowly cooled until th...

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PUM

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Abstract

The invention provides a method for separating the ursolic acid from the folium eriobotryae, which is characterized in that: having the folium eriobotryae as material, extracting in the water solution by the hydrophiled organic solvent, decompressed recovering the solvent, concentrating the folium eriobotryae total triterpenic acid extract; removing the impurities by solvent, active carbon decoloring, precipitation separation of the triterpenic acid extract to produce the coarse folium eriobotryae total triterpenic acid extract with the ursolic acid concentration >=25%; enrichment and purification, precipitation separation to the beige coarse ursolic acid with the ursolic acid concentration >=80%; extraction by hydrophilic organic solvent, crystalline purification and recrystallization of the coarse ursolic acid, and finally getting the purified ursolic acid crystal. The purity of the white ursolic acid crystal is >=99%. The invention has a simple producing process, a high separation efficiency, a low cost and a good extension.

Description

technical field [0001] The invention relates to a green chemical production method for extracting medicinal active components from plants, and more particularly to a method for separating and purifying ursolic acid from loquat leaves. Background technique [0002] China is the most important loquat producer in the world. The cultivated area and output of loquat account for more than 2 / 3 of the world's total. Its main producing areas are Putian in Fujian, Tangqi in Zhejiang and Dongting Mountain in Jiangsu. The existing loquat planting area in Putian, Fujian is 160,000 mu, accounting for about 1 / 6 of the world, 1 / 4 of the country and 1 / 2 of Fujian Province. [0003] Loquat is a treasure all over the body. Loquat leaves, flowers, fruits, seeds and other parts are rich in active ingredients such as triterpene acids (up to 1.5%), volatile oils, amygdalin, saponins, flavonoids, water-soluble polysaccharides and organic acids. At present, the supply of loquat is mainly based on f...

Claims

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Application Information

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IPC IPC(8): C07J63/00A61K36/73A61K127/00
Inventor 陈剑锋陈浩
Owner FUZHOU UNIV
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