Method for preparing encephalon glycoside and ignotin injection and the agent thereof

A technology of cerebroside carnosine and cerebroside carnosine, which is applied in the field of preparation of cerebroside carnosine, can solve the problems that the determination of the content will cause a certain impact, reduce the content of GM1 in the extract, and the pollution of polar esters is more serious. Avoid the possibility of allergic reactions, avoid content loss, reliable effect

Inactive Publication Date: 2007-09-26
BEIJING SAISHENG PHARMA
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  • Abstract
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  • Application Information

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Problems solved by technology

In the method of this application, due to the use of methanol-water mixed solution as the solvent for extracting GM1, the pollution of polar esters is relatively serious, which reduces the content of GM1 in the extract; the preparation contains excipients, and the determination of the content will be difficult Cause a certain impact, and cause the loss of polypeptide and GM1 during the freeze-drying process; and the excipients in the preparation are easy to form polymers with polypeptides and proteins during the preservation process, and the presence of biological macromolecules in patients will cause allergic reactions danger

Method used

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  • Method for preparing encephalon glycoside and ignotin injection and the agent thereof
  • Method for preparing encephalon glycoside and ignotin injection and the agent thereof

Examples

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preparation example Construction

[0055] Embodiments 1-3 are the preparation methods of the cerebroside carnosine solution, and Fig. 1 is the technical route and process flow chart for the preparation of the cerebroside carnosine solution of the present invention.

[0056] The left side of the accompanying drawing shows the preparation process of the animal brain extract solution, and the right side of the accompanying drawing shows the preparation process of the rabbit muscle extract solution.

Embodiment 1

[0059] Take 100Kg of quick-frozen pig brain and melt it in a special water tank, peel off the meningeal blood vessels, remove impurities and wash; homogenate the processed animal brain, add 4 times the amount of ice acetone to the homogenate, stir, place at -10°C, and centrifuge. Collect the precipitate; wash the precipitate 3 times with acetone, and dry to obtain about 5Kg of acetone powder;

[0060] Add 6 times the amount of chloroform-methanol mixture to acetone powder (the volume ratio of chloroform and methanol is 2:1), stir at room temperature, centrifuge, and collect the supernatant, which should be a clear organic solvent mixture; Repeat the extraction for more than 3 times, combine the supernatant, extract the total fat and concentrate by evaporation.

[0061] Folch partition the extracted total lipids in a 5% sodium chloride / methanol mixture (1:1, V / V), transfer the gangliosides from the organic phase to the aqueous phase, collect the upper layer, and repeat the dist...

Embodiment 2

[0082] Take 100Kg of quick-frozen sheep brain and melt it in a special water tank, peel off the meningeal blood vessels, remove impurities and wash; homogenate the processed animal brain, add 8 times ice acetone to the homogenate, place it at -10°C, centrifuge, and collect the precipitate The precipitate was washed 3 times with acetone, dried at 80°C to obtain 5Kg of acetone powder;

[0083] Add 10 times of chloroform-methanol mixture to acetone powder (the volume ratio of chloroform and methanol is 2:1), stir at room temperature, centrifuge, and collect the supernatant, which should be a clear organic solvent mixture; Extract more than 3 times, combine the supernatant, extract the total lipid and evaporate and concentrate.

[0084]Extract the total lipids and carry out Folch distribution in 5% sodium chloride / methanol mixture (1:1, V / V), so that gangliosides are transferred from the organic phase to the aqueous phase, the upper layer is collected, and the lower layer is repea...

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Abstract

The invention discloses a method for preparing cerebroside carnosine solution and relative cerebroside carnosine injection, wherein the cerebroside carnosine injection contains ganglioside and natural active peptide prepared by present biological purifying technique. And the inventive method comprises that using non-cow animal brain as material, using chloroform/carbinol mixture solvent to obtain the extractive solution with ganglioside from the brain, wherein the volume ratio of the chloroform/carbinol mixture solvent is 1-2:1, then preparing the extractive solution of small molecule peptide whose molecule weight is lower than 8000 from the muscle of non-cow animal, mixing the extractive solutions of step 1 and step 2, while the ganglioside: small molecule peptide is (85-115 mu g) :(5.44-7.36mg). The inventive injection can be used to treat cerebral infarction, coronary disease effectively or the like.

Description

technical field [0001] The present invention relates to the preparation method of cerebroside carnosine, more specifically, relates to the preparation method of cerebroside carnosine solution. [0002] The present invention also relates to the cerebroside-carnosine injection preparation prepared by the method of the present invention. Background technique [0003] Cerebroside and carnosine injection is a medicine containing compound gangliosides and natural active polypeptides purified and prepared by modern biotechnology. Ganglioside is a special substance that can directly act on human nerve cells and the central nervous system. The level of its content directly affects the growth, repair and regeneration of nerves, and has a high affinity for nerve tissues. Acid ganglioside (hereinafter referred to as ganglioside GM1 or GM1) is the most important ganglioside with high content in the body. The natural active peptides are mainly nerve growth factor (NGF) and neurotrophin-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/05A61K35/30A61K9/08A61P9/10A61K31/7032
Inventor 马骉单连慧姜桂荣
Owner BEIJING SAISHENG PHARMA
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