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Bacteria strain (KG strain) for culturing bait nematode and nematode culture method

A cultivation method and nematode technology, applied in the directions of bacteria, application, animal feed, etc., can solve the problems of large-scale cultivation of difficult biological bait, liquid cultivation is not as convenient as bacteria, etc., and achieve the effect of promoting industrialized cultivation and easy cultivation.

Inactive Publication Date: 2007-09-26
GUANGDONG ENTOMOLOGICAL INST
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

However, in commercial application, since Escherichia coli is a human pathogen, it is difficult to be used for large-scale cultivation of biological bait; although yeast is safe as a biological bait, the cultivation of yeast, especially liquid cultivation, is not as good as Bacteria are convenient

Method used

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Examples

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Embodiment Construction

[0012] The following experiments and operating examples are further illustrations of the present invention, and should not be regarded as limiting the present invention.

[0013] 1. Bacterial strain Xenorhabdus poinarii KG CCTCC M 206091 is contained on the peptone water medium containing 1% (mass percent) peptone and 0.5% (mass percent) sodium chloride, and is cultivated at 26° C. to obtain the bacterium liquid of the Xenorhabdus genus;

[0014] 2. The nematode medium was sterilized at 121° C. for 40 minutes. The composition ratio (by mass percentage) of the nematode medium was: yeast extract 1%, flour 10%, soybean powder 5%, lard 4%, water 65%, sponge 15%;

[0015] 3. Add 100 grams of sterilized nematode culture medium to a 500ml Erlenmeyer flask, then add the cultured Xenorhabdus bacterial solution, then insert sterile P.redivivus nematodes, and culture at 25-28°C , Harvest the cultured nematodes after 6 to 15 days, the nematodes in the culture can be harvested.

[0016] ...

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PUM

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Abstract

The invention discloses a bacteria strain (KG product system) to culture bait wire insect and culturing method, which comprises the following steps: choosing Xenorhabdus poinarii KG CCTCC M 206091 bacterial strain as producing bacterium; culturing the bacterial strain on protease leather water culture medium at 25-28 deg.c; getting bacterial liquid; adding into wire insect culture medium; switching in germ-free Panagrellus redivivus wire insect; placing under 25-28 deg.c; culturing 6-15 days; getting the culturing wire insect. This P.redivivus wire insect is safe for environment, human and animal, which is good biological bite of aquaculture.

Description

technical field [0001] The invention relates to a bacterium for cultivating bait nematodes and a method for cultivating the bait nematode Panagrellus redivivus. Background technique [0002] World-wide aquaculture, especially the aquaculture of prawns, has promoted the development of aquatic product seedling cultivation, and also intensified the demand for biological bait, especially the demand for Artemia. Artemia is generally collected from high-salinity waters, and its intensive farming has not yet formed a scale. Initially, the Great Salt Lake in Utah, USA was a place rich in Artemia. Over the years, the production of Artemia eggs has always accounted for more than 80% of the world's production, controlling the supply of the world market. However, since the 1990s, due to the over-harvesting of Artemia resources and changes in the salinity of the lake, the production of Artemia in salt lakes has declined sharply, resulting in high prices for Artemia. Central Asia also s...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/18A23K10/18A23K50/80
CPCY02A40/818
Inventor 韩日畴丘雪红曹莉刘小芬刘秀玲陈镜华
Owner GUANGDONG ENTOMOLOGICAL INST
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