Method for recombinant production of pancreatic glucagons sample peptide-2
A peptide sequence, tandem repeat technology, applied in the field of genetic engineering, can solve the problems of technical difficulty and high drug cost
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[0054] With the maturity of molecular biology technology, gene recombinant polypeptide is becoming an important way of drug research and development. Compared with peptide synthesis, genetic recombination has the following advantages: 1. High biological activity; 2. Economical, the cost is less than 10% of peptide synthesis; 3. Easy molecular modification to make its effect more obvious. For GLP-2, its molecular weight is only 3.9kDa, so it is difficult to express it by common gene recombination. In a specific embodiment of the present invention, we designed the following method to make expression and purification easier. Its characteristics are:
[0055] 1. Select the pET31 protein expression system that is more suitable for the expression of small molecule polypeptides, and design 3 copies of GLP-2, so that the molecular weight of its expression can reach 24kDa, which is the most suitable molecular weight range for protein expression. 3 copies can make GLP-2 obtain rate is...
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