Method for recombinant production of pancreatic glucagons sample peptide-2

A peptide sequence, tandem repeat technology, applied in the field of genetic engineering, can solve the problems of technical difficulty and high drug cost

Inactive Publication Date: 2007-09-26
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, the cost of peptide synthesis drugs is relatively high, and the synthesis of peptides with >30 amino acids is also technically difficult

Method used

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  • Method for recombinant production of pancreatic glucagons sample peptide-2
  • Method for recombinant production of pancreatic glucagons sample peptide-2
  • Method for recombinant production of pancreatic glucagons sample peptide-2

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Embodiment Construction

[0054] With the maturity of molecular biology technology, gene recombinant polypeptide is becoming an important way of drug research and development. Compared with peptide synthesis, genetic recombination has the following advantages: 1. High biological activity; 2. Economical, the cost is less than 10% of peptide synthesis; 3. Easy molecular modification to make its effect more obvious. For GLP-2, its molecular weight is only 3.9kDa, so it is difficult to express it by common gene recombination. In a specific embodiment of the present invention, we designed the following method to make expression and purification easier. Its characteristics are:

[0055] 1. Select the pET31 protein expression system that is more suitable for the expression of small molecule polypeptides, and design 3 copies of GLP-2, so that the molecular weight of its expression can reach 24kDa, which is the most suitable molecular weight range for protein expression. 3 copies can make GLP-2 obtain rate is...

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Abstract

The invention discloses a preparing method of glucagons example peptide -2(GLP-2) through restructuring, which can prepare a good deal of stable biological active GLP-2 peptide. This invention also discloses a usage to prepare drugs for accelerate growth of intestine mucosa with GLP-2 peptide.

Description

technical field [0001] The present invention generally relates to the field of genetic engineering, and specifically, the present invention relates to a method for recombinantly producing glucagon like peptide-2 (GLP-2). Background technique [0002] 1. Biological properties of glucagon like peptide-2 (GLP-2) [0003] GLP-2 is one of the products expressed by the proglucagon gene (PG), which belongs to the proglucagon-derived peptides (PGDP). PG has 6 exons, including A cAMP response element and an upstream enhancer of glucagon, the gene product encoded by PG is characterized by being mainly expressed in the pancreas and gastrointestinal tract, and produces different peptides, such as glucagon with amino acid sequence 1-69, GLP-1 at positions 78-107, glucagon-related pancreatic polypeptide (GRPP) at positions 1-30, and GLP-2 at positions 126-158 have various biological effects. Among them, GLP-2 is synthesized and secreted by intestinal L cells, wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/09C12N15/12C12N15/63C12N1/21C07K14/435C07K14/605A61K38/17A61K38/26A61P1/00
Inventor 赵云王凤君汪仕良黄跃生
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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