Immunogen by using mutant CRM197 of diphtheria toxin as carrier, preparation method, and application

A technology of CRM197 and G17CRM197, which is applied in the field of immunogen G17CRM197 and its preparation, can solve the problems of not reaching the theoretical cross-linking rate and the difference in the cross-linking rate of sugars
CN101050236AActive Publication Date: 2007-10-10QILU PHARMA HAINAN
1 Cites 17 Cited by

Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
QILU PHARMA HAINAN
Publication Date
2007-10-10

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

This invention relates to a method for preparing immunogen G17CRM197 containing diphtherin mutant CRM197 as carrier. The method comprises: crosslinking diphtherin mutant CRM197 with gastrin G17 through epsiv-maleimidyl caproic acid N-hydroxysuccinimide eater (EMCS). Diphtherin mutant CRM197 is used as a carrier, and has such advantages as high recovery rate and easy purification. The method has such advantages as high product crosslinking rate, short preparation time, and low cost. Immunogen G17CRM197 can be used as an effective component in therapeutic vaccines, or used in anti-tumor drugs with appropriate immunoadjuvants.
Need to check novelty before this filing date? Find Prior Art

Description

(1) Technical field

[0001] The invention relates to an immunogen G17CRM197 with diphtheria toxin mutant CRM197 as a carrier and a preparation method thereof, as well as the application of the immunogen in the treatment of digestive tract tumors, belonging to the technical field of medicine. (2) Background technology

[0002] CRM197 (cross-reacting materials 197) is a mutant that loses the toxicity of diphtheria toxin, see Wu Qida et al., Diphtheria toxin and related proteins I. Isolation and description of diphtheria toxin serotype-related mutant strains, Biochemistry, 1973.248 : 3838-3844. (Uchida, T., A.M.Pappenheimer, Jr.and R.Gregory.al., Diphtheria toxin and related proteins I.Isolation and properties of mutantproteins serologically related to diphtheria toxin.J.Biol.Chem.1973.248: 3838-3844.), which is mutated from a base G to A in the base sequence of the wild-type diphtheria toxin, thus resulting in the mutation of the 52nd amino acid GLY to GLU, see Giannini et al.,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More