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Cell-eliminating coanea matrix and its prepn process

A corneal stroma and decellularization technology, applied in the field of medical devices, can solve the problems of unsuitable corneal stroma deep lesion repair, unfavorable cell nutrient diffusion, high rate of aseptic inflammatory reaction, etc., to inhibit the growth of new blood vessels, tissue phase Good capacity and easy operation

Inactive Publication Date: 2007-11-07
ZHONGSHAN OPHTHALMIC CENT SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Natural polymer materials such as collagen, hyaluronic acid, chitin and its derivatives, and polysaccharide polymers have poor mechanical properties, are too hydrophobic, and degrade too quickly, which is not conducive to the diffusion of cell nutrients into the material.
Artificially synthesized polymer materials (such as polylactic acid and polyglycolic acid) are also used as carriers for tissue engineering corneas. Their transparency and stability are poor, and the rate of aseptic inflammatory reactions is high, so they are not suitable for clinical trials.
Although amnion tissue can provide a continuous basement membrane, containing collagen, laminin, fibronectin and various integrins, these components are conducive to the differentiation and migration of epithelial cells, but the material itself is too thin and not suitable for the repair of deep lesions in the corneal stroma

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A decellularized corneal stroma is mainly composed of hundreds (about 100-250) collagen fiber laminae parallel to each other, and there are gaps formed after the cells are removed between the collagen fiber laminae. The collagen fiber laminae are parallel to each other, with the same size and equal intervals. The gap between the collagen fiber laminae also contains corneal stroma (including mucopolyprotein and glycoprotein, etc.) and remaining cell membrane and cytoplasmic components after decellularization. The shape of the decellularized corneal stroma is regular, and its thickness is about 0.1-0.5mm (the specific shape and specification depend on the clinical case).

[0030] The preparation method of decellularized corneal stroma adopts heterogeneous animal corneal stroma as raw material, and can be obtained through decellularization by chemical infiltration, and the steps are as follows:

[0031] Preparation of acellular corneal matrix by chemical osmosis: Treat het...

Embodiment 2

[0040] A decellularized corneal stroma, composed of hundreds of parallel collagen fiber lamellae, with spaces between the collagen fiber lamellae formed by the decellularization of cells. Collagen fiber laminae are parallel to each other, with the same size and equal intervals, and its basic components include collagen fibers, corneal stroma (including mucopolyprotein and glycoprotein, etc.), and residual membrane and cytoplasmic components after decellularization. The decellularized corneal stroma is a circle with a diameter of about 1-10 mm and a thickness of about 0.3 mm. (The specific diameter and thickness can be shaped according to the refractive status and pathological changes of the affected eye)

[0041] The preparation method of decellularized corneal stroma adopts the allogeneic corneal stroma as raw material, and the cornea includes the sclera and conjunctiva attached to the cornea, which can be obtained through decellularization by chemical osmosis, and the steps ...

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Abstract

The present invention discloses one kind of cell-eliminating cornea matrix, which consists of mainly hundreds of parallel collagen fiber plate layers with voids formed through eliminating cells in between. The cell-eliminating cornea matrix is used in repairing, reconstructing and shaping surficial eye defect, treating surficial eye diseases and constructing tissue engineering cornea. After being transplanted, the cell-eliminating cornea matrix may exist for long term or degrade gradually, and is high transparent. It may allow receptor nerve to grow into and receptor cell to attach on, and has the features of low immunogenicity and high tissue compatibility.

Description

technical field [0001] The invention belongs to the field of medical devices, and in particular relates to a decellularized corneal stroma and a preparation method thereof. Background technique [0002] Corneal transplantation is one of the important methods for the treatment of corneal diseases, defects, opacity, and abnormal refractive power caused by various causes such as infection, trauma, and congenital abnormalities. Due to the lack of donor sources, many patients cannot receive timely and effective treatment. Due to the poor biocompatibility of artificial corneas made of synthetic materials, it is difficult to fuse with the surrounding tissues after transplantation, and complications such as detachment, water leakage, and anterior and posterior membranes are prone to occur. [0003] Pterygium is a common ocular surface disease that is prone to recurrence after surgery. Simple pterygium excision combined with limbal transplantation can reduce the postoperative recurre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/54A61F2/14
CPCA61L27/38A61L27/3633A61L27/3641A61L2430/16
Inventor 王智崇
Owner ZHONGSHAN OPHTHALMIC CENT SUN YAT SEN UNIV
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