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A kind of preparation method and application of agarose/nano-silver composite gel

A composite gel and agarose technology, applied in the fields of botanical equipment and methods, applications, nanotechnology, etc., can solve the problems of lack of support, the release speed cannot be effectively controlled, etc., and achieve good bacteriostatic effect and good scaffold. effect, good compatibility

Active Publication Date: 2019-02-01
武汉勿幕科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These nano-silver materials themselves do not have a supporting function, and the release of nano-silver in the material is very fast, and the release rate cannot be effectively controlled.

Method used

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  • A kind of preparation method and application of agarose/nano-silver composite gel
  • A kind of preparation method and application of agarose/nano-silver composite gel
  • A kind of preparation method and application of agarose/nano-silver composite gel

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preparation example Construction

[0031] Such as figure 1 Shown, the preparation method of the agarose / nano-silver composite gel of the embodiment of the present invention comprises the following steps:

[0032] S01: providing a first solution, wherein the first solution comprises agarose dispersed in a first solvent;

[0033] S02: providing a second solution, wherein the second solution comprises a silver salt dispersed in a second solvent;

[0034] S03: Mixing the first solution and the second solution to obtain the agarose / nano-silver composite gel.

[0035] The agarose powder is dispersed and dissolved in the first solvent, and heated and stirred to form a transparent and uniform solution; the first solvent can be water, or the effect is better when ultrapure water is used.

[0036] The silver salt may be silver nitrate or silver acetate, and the second solvent for dissolving and dispersing the silver nitrate or silver acetate may be water.

[0037] The process of mixing the first solution and the secon...

Embodiment 1

[0044] Example 1 of the present invention The preparation method of agarose / nano-silver composite gel and its bacteriostatic application comprises the following steps: taking 0.21 g of agarose powder and adding it to 6.0 mL of ultrapure water, heating and stirring to dissolve the agarose powder, A clear homogeneous solution was formed. Add 4.0 mL of 0.5 M silver nitrate solution to the above agarose solution, and stir at a constant temperature of 100° C. for 15 min. After stopping the heating, the mixed solution was placed at 20° C. for 7 days to obtain an agarose / nano-silver composite gel. Place the agarose / nanosilver composite gel in a concentration of 10 5 cfu / mL Escherichia coli LB medium, sealed and placed in a constant temperature incubator at a temperature of 37°C, and the medium was taken out after 24 hours.

[0045] The preparation steps of each liter of LB medium are as follows: add 10 g of tryptone, 5 g of yeast extract, 10 g of NaCl, and 15 g of agar powder into 95...

Embodiment 2

[0048] Example 2 of the present invention The preparation method of agarose / nano-silver composite gel and its antibacterial application include the following steps: take 0.15g of agarose powder and add it to 7.2mL of ultrapure water, heat and stir to dissolve the agarose powder, A clear homogeneous solution was formed. Add 2.8 mL of silver nitrate solution with a concentration of 1.0 M to the above solution, and stir at a constant temperature of 100° C. for 1 min. After stopping the heating, the mixed solution was placed at 30° C. for 15 days to obtain an agarose / nano-silver composite gel. Place the agarose / nanosilver composite gel in a concentration of 10 5 cfu / mL Staphylococcus aureus LB medium, sealed and placed in a constant temperature incubator with a temperature of 37°C, and the medium was taken out after 24 hours. Figure 4 It is the ultraviolet-visible absorption spectrum figure of the agarose / nanometer silver composite gel of embodiment 2. Figure 5 It is the anti...

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Abstract

The invention provides a preparation method and an application of agarose / nano-silver composite gel. The preparation method of the agarose / nano-silver composite gel comprises steps as follows: providing a first solution comprising agarose dispersed in a first solvent; providing a second solution comprising silver salt dispersed in a second solvent; mixing the first solution and the second solution to obtain the agarose / nano-silver composite gel. The agarose / nano-silver composite gel can be applied as a bacteriostat and has good bacteriostasis performance, and nano-silver particles are released slowly.

Description

technical field [0001] The invention belongs to the technical field of nano-metal material preparation, and in particular relates to a preparation method and application of agarose / nano-silver composite gel. Background technique [0002] Due to its small size effect and surface effect, nano silver has strong penetrating power and antibacterial activity. It has the characteristics of broad-spectrum antibacterial, strong bactericidal, strong permeability, long-lasting antibacterial and no drug resistance, repair and regeneration ability, so it has been widely used in biomedicine and antibacterial materials. [0003] It has been reported that using polyethylene glycol as a protective agent, using photocatalytic reduction method, and using ultraviolet light to stimulate photoelectron reduction of silver nitrate to prepare a nano-silver stock solution with a particle size of 1nm to 100nm, after mixing this stock solution with various additives A fluid nano-silver antibacterial l...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B22F9/24B82Y40/00A01N25/10A01N59/16A01P1/00
CPCA01N25/10A01N59/16B22F9/24B82Y40/00
Inventor 吴江渝杨婉李竹肖文雅曾小平陈喆张勇
Owner 武汉勿幕科技有限公司
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