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Cell-eliminating coanea matrix and its preparation process

A corneal stroma and decellularization technology, which is applied in the field of medical devices, can solve the problems of unsuitable corneal stroma deep lesion repair, unfavorable cell nutrient diffusion, high rate of aseptic inflammatory reaction, etc., to alleviate the shortage of donor materials, tissue phase Good capacity and the effect of inhibiting the growth of new blood vessels

Inactive Publication Date: 2010-05-19
ZHONGSHAN OPHTHALMIC CENT SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Natural polymer materials such as collagen, hyaluronic acid, chitin and its derivatives, and polysaccharide polymers have poor mechanical properties, are too hydrophobic, and degrade too quickly, which is not conducive to the diffusion of cell nutrients into the material.
Artificially synthesized polymer materials (such as polylactic acid and polyglycolic acid) are also used as carriers for tissue engineering corneas. Their transparency and stability are poor, and the rate of aseptic inflammatory reactions is high, so they are not suitable for clinical trials.
Although amnion tissue can provide a continuous basement membrane, containing collagen, laminin, fibronectin and various integrins, these components are conducive to the differentiation and migration of epithelial cells, but the material itself is too thin and not suitable for the repair of deep lesions in the corneal stroma

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] An acellular corneal stroma, which is mainly composed of hundreds (about 100-250) collagen fiber lamellae that are parallel to each other, and there are gaps formed after the cells are removed between the collagen fiber lamellae. Collagen fiber lamellae are parallel to each other, with the same size and equal spacing. The gaps between the collagen fiber lamellae also contain corneal stroma (including mucopolyproteins and glycoproteins, etc.), as well as residual membrane and cytoplasmic components after decellularization. The acellular corneal stroma has a regular shape, and its thickness is about 0.1-0.5 mm (the specific shape and specification depend on the clinical case).

[0032] The preparation method of acellular corneal stroma is obtained by taking xenogeneic animal corneal stroma as a raw material and decellularized by chemical osmosis, and the steps are as follows:

[0033] Decellularized corneal stroma prepared by chemical osmosis: Treat the xenogeneic corneal...

Embodiment 2

[0042] An acellular corneal stroma consisting of hundreds of parallel layers of collagen fibers with spaces between the layers of collagen fibers formed after cells have been sloughed off. Collagen fiber lamellae are parallel to each other, with the same size and equal interval, and its basic components include collagen fibers, corneal stroma (including mucopolyprotein and glycoprotein, etc.), as well as residual membrane and cytoplasmic components after decellularization. The decellularized corneal stroma is a circle with a diameter ranging from about 1 to 10 mm, and a thickness of about 0.3 mm. (The specific diameter and thickness can be shaped according to the refractive state and pathological condition of the affected eye)

[0043] The preparation method of acellular corneal stroma adopts allogeneic corneal stroma as raw material, the cornea includes sclera and conjunctiva attached to the cornea, and can be obtained by decellularization treatment by chemical osmosis, and t...

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Abstract

The present invention discloses one kind of cell-eliminating cornea matrix, which consists of mainly hundreds of parallel collagen fiber plate layers with voids formed through eliminating cells in between. The cell-eliminating cornea matrix is used in repairing, reconstructing and shaping surficial eye defect, treating surficial eye diseases and constructing tissue engineering cornea. After beingtransplanted, the cell-eliminating cornea matrix may exist for long term or degrade gradually, and is high transparent. It may allow receptor nerve to grow into and receptor cell to attach on, and hasthe features of low immunogenicity and high tissue compatibility.

Description

technical field [0001] The invention belongs to the field of medical devices, in particular to a decellularized corneal stroma and a preparation method thereof. Background technique [0002] Corneal transplantation is one of the important methods for the treatment of corneal diseases, defects, opacities, and refractive abnormalities caused by various causes such as infection, trauma and congenital anomalies. Due to the lack of donor sources, many patients cannot receive timely and effective treatment. Due to poor biocompatibility, artificial corneas made of synthetic materials are difficult to fuse with periocular tissues after transplantation, and are prone to complications such as loss, water leakage, and anterior and posterior membranes. [0003] Pterygium is a common ocular surface disease with the characteristics of easy postoperative recurrence. Simple pterygium excision combined with corneal limbal transplantation can reduce the postoperative recurrence rate of the di...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/54A61F2/14
CPCA61L27/3633A61L27/38A61L2430/16A61L27/3641
Inventor 王智崇
Owner ZHONGSHAN OPHTHALMIC CENT SUN YAT SEN UNIV
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