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Decellularized eyelid board and preparation method thereof

A decellularization and meibomian technology, applied in the field of medical devices, can solve the problems of being unsuitable for eyelid construction, unable to restore the secretion function of meibomian glands, poor histocompatibility and stability, etc., to inhibit the growth of new blood vessels, alleviate the The effect of insufficient donor material and good histocompatibility

Inactive Publication Date: 2013-06-05
ZHONGSHAN OPHTHALMIC CENT SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Natural polymer materials such as collagen, hyaluronic acid, chitin and its derivatives, and polysaccharide polymers have poor mechanical properties, are too hydrophobic, and degrade too quickly, which is not conducive to the diffusion of cell nutrients into the material.
Artificially synthesized polymer materials (such as polylactic acid and polyglycolic acid) are also used as carriers for tissue engineering eyelids. Their histocompatibility and stability are poor, and the rate of aseptic inflammatory reactions is high, so they are not suitable for clinical use. test
Although amnion tissue can provide a continuous basement membrane, containing collagen, laminin, fibronectin and various integrins, these components are conducive to the differentiation and migration of epithelial cells, but the material itself is too thin and not suitable for constructing eyelids; allogeneic sclera, Acellular dermis, dura mater, fascia, ligament, and cartilage are also used as eyelid substitutes, but they are easy to be lost, melted, degraded, and exposed, especially without meibomian ducts and meibomian glands. Gland secretion function recovery

Method used

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  • Decellularized eyelid board and preparation method thereof
  • Decellularized eyelid board and preparation method thereof
  • Decellularized eyelid board and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Preparation of decellularized tarsus by chemical osmosis: take the tarsus of rabbits, soak them in an anionic surfactant SDS aqueous solution with a concentration of 0.1% by mass, and treat the tarsus at 37°C for 7 hours to obtain decellularized tarsus . Paraffin sections were made for histopathological observation, the results showed that the cellular components in the matrix were removed, and the tissue observation showed, such as figure 1 As shown, the decellularized tarsus includes tarsus 1 on both sides, several collagen fiber laminae 2 running toward each other between the tarsus, 20-50 meibomian gland ducts 3 between the collagen fiber laminae, and collagen There are also gaps formed after the cells are removed between the fiberboard layers. The basic components of the decellularized meibomian include collagen fibers, meibomian matrix (including mucopolyprotein and glycoprotein, etc.), meibomian gland ducts, and remaining membrane and cytoplasmic components aft...

Embodiment 2

[0052] Chemiosmotic method to make decellularized tarsus: Take the tarsus of pig, which is attached with the skin, muscle and conjunctiva on the eyelid tissue, soak it in the detergent Triton X-100 aqueous solution with a concentration of 1% by mass In this method, the tarsus was treated at 37°C for 24 hours to obtain decellularized tarsus. Paraffin sections were made for histopathological observation, the results showed that the cellular components in the matrix were removed, and the tissue observation showed, such as figure 1 As shown, the decellularized tarsus includes tarsus 1 on both sides, several collagen fiber laminae 2 running toward each other between the tarsus, 20-50 meibomian gland ducts 3 between the collagen fiber laminae, and collagen There are also gaps formed after the cells are removed between the fiberboard layers. The basic components of the decellularized meibomian include collagen fibers, meibomian matrix (including mucopolyprotein and glycoprotein, et...

Embodiment 3

[0057] Preparation of acellular tarsus by chemical osmosis: take the tarsus of a monkey, soak it in an anionic surfactant SDS aqueous solution with a concentration of 0.05% by mass, and treat the tarsus at 45°C for 4 hours to obtain acellular tarsus . Paraffin sections were made for histopathological observation, the results showed that the cellular components in the matrix were removed, and the tissue observation showed, such as figure 1 As shown, the decellularized tarsus includes tarsus 1 on both sides, several collagen fiber laminae 2 running toward each other between the tarsus, and 1 to 50 meibomian gland ducts 3 between the collagen fibrous laminae. There are also gaps formed after the cells are removed between the fiberboard layers. The basic components of the decellularized meibomian include collagen fibers, meibomian matrix (including mucopolyprotein and glycoprotein, etc.), meibomian gland ducts, and remaining membrane and cytoplasmic components after decellulariz...

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Abstract

The invention discloses a decellularized eyelid board and a preparation method thereof. The accellular eyelid board adopts an eyelid board of a heterogeneous animal or an allogeneic eyelid board as a raw material which is subjected to decellularization treatment to obtain the decellularized eyelid board. The accellular eyelid comprises eyelid board bodies on two sides. A plurality of collagen fibreboard layers which are respectively arranged in the crossing directions are arranged between the eyelid board bodies. A plurality of meibomian gland tubes are arranged between the collagen fibreboard layers. Spaces formed after cells are removed are also formed between the collagen fibreboard layers. The decellularized eyelid board can be used for repairing defects and reestablishment of eyelid tissues, shaping the eyelid tissues and treating eyelid diseases, and can be used as a carrier for building a tissue-engineering biological eyelid board. The decellularized eyelid board can exist in a long term after being transplanted and has the functions of meibomian glands of a receptor. Nerves, blood vessels and lymph vessels can grow into the decellularized eyelid board, and cells of the receptor can be attached to and grow on the surface of the decellularized eyelid board in a long term. The decellularized eyelid board has the advantages of being low in immunogenicity and good in histocompatibility, and having wide application prospect.

Description

technical field [0001] The invention belongs to the field of medical devices, and in particular relates to a decellularized tarsus and a preparation method thereof. Background technique: [0002] Eyelid diseases and defects caused by various causes such as tumor, infection, trauma and congenital abnormalities, and then cause secondary damage to ocular surface tissues to varying degrees, such as entropion and trichiasis, exposure keratitis, corneal ulcer, etc. Due to the current The meibomian substitute can only provide the meibomian structure without the meibomian glands, so many patients have different degrees of meibomian gland functional defects after operation, which cannot be effectively treated. In addition, tarsus substitutes made of synthetic materials have poor biocompatibility and are difficult to fuse with the surrounding tissues after transplantation, and are prone to complications such as loss, exposure, melting, and infection. [0003] Trachoma is a common ocu...

Claims

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Application Information

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IPC IPC(8): A61L27/36
Inventor 梁轩伟王忠浩王智崇黄丹平周强
Owner ZHONGSHAN OPHTHALMIC CENT SUN YAT SEN UNIV
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