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Process of identifying karst plant by means of carbonic anhydrase activity

A technique for measuring carbonic anhydrase and activity, which is applied in the fields of afforestation technology and ecological management, can solve the problems of expensive equipment, complicated operation, and high testing cost, and achieve the effect of low cost, simple operation, and favorable promotion

Inactive Publication Date: 2010-11-17
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

Radioimmunoassay has high sensitivity and good reproducibility, but the operation is complicated; isotope CO 2 Mass spectrometry, high sensitivity, simple operation, good reproducibility, but expensive equipment; colorimetry, fluorescence photometry, high accuracy, but high testing cost

Method used

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Experimental program
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Embodiment Construction

[0011] Platycarya Longipes Wu., Populus tomentosa Carr., Salix babylonica L., Broussonetia papyrifera (Linn.) Vent., Morus mongolica Schneid., Mulberry (Morus alba L), Maple Poplar (Pterocarya stenopteraD.DC.), Neem (Melia azedarach Linn.), Peach (Prunus persica Batsch.), Yellow Vitex (Vitexnegundo Linn.), Rendou (Zenia insigins Chun), Huaihuai Tree (Sophora japonica L.), tomato (Lycopersicum esculentum Mill), Chinese cabbage (Brassica chinensis L.), rice chestnut (Cardamine hirsuta L.), blue flower seed (Raphanus sativa L. var. raphanistroides Makino), Ethiopian mustard (Brassica carinata A. Braun), Atractylodeslancea (thunb.) DC.) as candidate plants. Take them and the normal unfolded leaves in the vegetative growth period of Zhuge Cai to measure the activity of carbonic anhydrase, and the determination adopts the commonly used pH meter method. Calculate the ratio of the carbonic anhydrase activity value of the leaf of the alternative plant to the carbonic anhydrase activit...

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Abstract

The process of identifying karst adaptive plant by means of carbonic anhydrase activity relates to forest plantation technology and ecological treatment technology. The process includes taking the living candidate plant material in vegetative growth period and health leaf of Orychophiagmus violaceus, detecting their carbonic anhydrase activity separately, comparing and selecting the plant with leaf possessing carbonic anhydrase activity higher than 50 % of that of Orychophiagmus violaceus as the karst adaptive plant. In this way, karst adaptive plant may be selected easily in low cost.

Description

technical field [0001] The invention relates to the fields of afforestation technology and ecological management, in particular to a method for identifying karst plants suitable for growth by using carbonic anhydrase activity. Background technique [0002] In karst areas, due to a large amount of surface water leakage, exposed rocks, barren soil, and the lack of semi-weathered parent material layer, the distribution of water resources is uneven, and the soil's water holding capacity is low, which is not conducive to the growth of plant roots. Most plants grow under these water and soil conditions. slow, resulting in low vegetation coverage in this area. However, some plants can grow well under these conditions and have strong adaptability, forming a contiguous primary karst forest of more than 20,000 hectares in Maolan. [0003] There are various mechanisms for plants in karst areas to adapt to drought conditions. Coniferous plants, some plants with thicker cuticles or waxy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/527
Inventor 吴沿友刘从强李萍萍周文美王宝利
Owner JIANGSU UNIV