Production and use for algin oligose
A technology of alginate oligosaccharide and alginate, applied in the directions of oligosaccharides, fungi, etc., can solve the problems of chemical substance residue, high cost, difficult control of process conditions, etc., and achieves low cost, less equipment, and analgesic effect. Effect
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Embodiment 1
[0016] Algin oligosaccharides are prepared according to the following method: Take 5.0 g of algin solid with a viscosity average molecular weight of 128,000, and use 2.5% acetic acid solution to prepare 400 mL of 1.25% algin solution. When the temperature is 60 ° C and the pH value is 4.0 When the value is high, use 150 Hz ultrasonic wave under the condition of 80% power to carry out ultrasonic degradation for 8 hours, add absolute ethanol to the degradation solution for alcohol analysis, filter with suction, dry, and grind to obtain 4.6 g of alginate oligosaccharides. Its average degree of polymerization is 8.
Embodiment 2
[0018] Alginate oligosaccharides are prepared according to the following method: Take 12.0 g of alginate solids with a viscosity-average molecular weight of 128,000, and use 2.5% acetic acid solution to prepare 400 mL of 3.0% alginate solution. When the temperature is 80 ° C and the pH value is 3.0 10.5 g of algin oligosaccharides was obtained by adding anhydrous ethanol to the degradation solution for alcohol analysis, suction filtration, drying, and grinding. Its average degree of polymerization was 10.
[0019] The following is a detailed description of the experimental method and experimental results that algin oligosaccharides can be used as natural antibacterial agents with high antibacterial activity.
[0020] The screening and effect evaluation methods of the prepared alginate oligosaccharide antibacterial test are as follows:
[0021] Using 2.5% alginate, 2.5% sodium benzoate and normal saline as controls, the antibacterial effect of 2.5% alginate oligosaccharide sol...
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