Method for producing paeonin metabolite-I by short lactobacillin fermentation

A technology of paeoniflorin metabolites and Lactobacillus brevis, which is applied in the field of biochemical engineering, can solve the problems of unsuitability for industrialization, no report of yield rate, long conversion time, etc., and achieve easy control of the process, mature separation method, and fast production cycle Effect
CN101074447AInactive Publication Date: 2007-11-21CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
Publication Date
2007-11-21
Estimated Expiration
Not applicable · inactive patent

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Abstract

A method for producing paeonoside metabolic hormone-I by fermenting short Lactobacillus is carried out by inducing L.brevis AS 1.12 into culture medium with tomato juice 200g / L, protein peptone 7.5g / L, yeast paste 7.5g / L, glucose 10g / L, Tween-80 0.3 ml / L and pH=7.0-8.5, anaerobic culturing at 25-37 degree for 12-24 hrs, centrifugal collecting bacterial cells, washing by normal saline, centrifuging, dispersing obtained cells into buffer liquid of 0.05M phosphate, adding into substrate paeonoside or its analogs hydroxy-paeonoside or methyl-paeonoside or benzoyl-paeonoside or mixture of paeonoside and its analogs, converting at 30-40 degree for 2-24 hrs, extracting by ethyl acetate, drying concentrated extract to obtain concrete, chromatographying by silica-gel column to obtain final product. It's simple, cheap, efficient, fast and controllable and has friendly environment. It can be used for anti-inflammatory, stress ulcer, analgesic and antispasmodic, ecstatic coronary blood vessel, myocardial ischemia and platelet aggregation-inhibition.
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Description

technical field

[0001] The invention belongs to the field of biochemical engineering, and relates to a method for producing paeoniflorin metabolite-I by fermenting lactobacillus. Background technique

[0002] Paeoniflorin is the main active ingredient of Paeoniflorin, which has various effects such as analgesic and antispasmodic, anti-inflammatory, anti-stress ulcer, expansion of coronary vessels, anti-acute myocardial ischemia and inhibition of platelet aggregation. Recent studies have shown that after oral administration of paeoniflorin, there is little direct absorption and low bioavailability, suggesting that paeoniflorin is first degraded by intestinal bacteria in the intestinal tract, and its metabolites play the pharmacological role of paeoniflorin. Japan's Masao Hattori et al. first reported that paeoniflorin was converted into paeoniflorin metabolin-I, II, III (see Figure 1 for Paeonimatabolin-I, II, III) in human intestinal bacteria in v...

Claims

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