Magnetic chitosan medicine-carried nano particles and preparation method thereof

A drug-loading nanometer and nanoparticle technology, which is used in drug combinations, antitumor drugs, inorganic non-active ingredients, etc., can solve the problems of poor dispersion and large particle size, and achieve the effect of good dispersion and no agglomeration phenomenon.

Inactive Publication Date: 2007-12-12
SHANGHAI NORMAL UNIVERSITY
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Problems solved by technology

[0005] From the current research on chitosan in the preparation of magnetic drug-loaded nanoparticles, there are generally problems such as poor dispersion and large particle size (mostly micron-sized particles) (He Qiangfang, Li Guoming, Wu Haizhen, etc. Applied Chemistry 2004, 21(02): 192~196)

Method used

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  • Magnetic chitosan medicine-carried nano particles and preparation method thereof
  • Magnetic chitosan medicine-carried nano particles and preparation method thereof

Examples

Experimental program
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Embodiment 1

[0023] Using the emulsion-crosslinking method, using chitosan for 5-fluorouracil and γ-Fe 2 o 3 The nanoparticles are wrapped to obtain spherical particles with a particle size of about 70-130nm. The specific steps are as follows:

[0024] (1), 100mg chitosan, 0.1mg γ-Fe 2 o 3 Nanoparticles (average particle diameter 20nm) mix with 0.5% dilute acetic acid solution (mass ratio) 2ml containing 40mg 5-fluorouracil, join in the 40ml liquid paraffin (volume ratio) that has been dissolved with 0.5% Span 80, in Stir at constant temperature for 2 hours at 35°C;

[0025] (2) Add 0.5ml of 2% trisodium citrate to the mixture, react for 30 minutes and solidify and precipitate particles; wash the obtained particles with petroleum ether and isopropanol respectively, and dry and store the particles in vacuum.

[0026] Particle characterization was carried out with testing instruments such as infrared spectrometer (FT-IR) and transmission electron microscope (TEM). The results are shown ...

Embodiment 2

[0030] (1), the chitosan of 120mg, 0.2mg γ-Fe 2 o 3 Nanoparticles (average particle diameter 20nm) mix with 0.5% dilute acetic acid solution (mass ratio) 2ml containing 50mg 5-fluorouracil, join in the 35ml liquid paraffin (volume ratio) that has been dissolved with 0.5% Span 80, in Stir at constant temperature for 2 hours at 35°C.

[0031] (2) Add 0.5ml of 2% trisodium citrate to the mixture, react for 30 minutes and solidify and precipitate particles; wash the obtained particles with petroleum ether and isopropanol respectively, and dry and store the particles in vacuum.

Embodiment 3

[0033] (1), 180mg chitosan, 0.1mg γ-Fe 2 o 3 Nanoparticles (average particle diameter 20nm) mix with 1% dilute acetic acid solution (mass ratio) 2ml containing 100mg 5-fluorouracil, join in the 40ml liquid paraffin (volume ratio) that has been dissolved with 0.4% Span 80, in Stir at constant temperature for 2 hours at 40°C. All the other steps are the same as in Example 1.

[0034] (2) Add 0.5ml of 2% trisodium citrate to the mixture, react for 30 minutes and solidify and precipitate particles; wash the obtained particles with petroleum ether and isopropanol respectively, and dry and store the particles in vacuum.

[0035] In Examples 1-3, the obtained CS / 5-Fu MNPs were dissolved in 0.1 mol / L hydrochloric acid solution, and the encapsulation efficiency of 5-Fu was determined to be 40%-50%, and the drug loading capacity of the particles was 15 %-25%.

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Abstract

The invention relates to a magnetic chitosan drug-loaded nanoparticle and its preparation method. Magnetic chitosan drug-loaded nanoparticle is spheroidal particle with particle diameter of 70-130nm and 5-flurouracil as active component. The preparation method comprises adopting acetic acid aqueous solution containing chitosan, gamma -Fe2O3nanoparticle and 5-flurouracil as water phase and liquid paraffin containing surfactant Span 80 as oil phase, mixing water phase and oil phase to obtain micro emulsion, washing with sodium citrate as cross-linking agent, and drying to obtain magnetic chitosan drug-loaded nanoparticle with entrapment efficiency of 40-50%, and drug loading dosage of particle of 15-25%.The inventive magnetic drug-loaded nanoparticle has obvious effects in promoting apoptosis of tumor cell, nano-graded particle diameter, good dispersity, no coacervation, and sustained release effect.

Description

technical field [0001] The invention relates to the field of biomedical drug carriers, in particular to chitosan drug-loaded particles, in particular to magnetic chitosan drug-loaded nanoparticles. technical background [0002] Chitosan is the product of deacetylation of chitin. It is an aminopolysaccharide with many unique physical, chemical properties and biological functions. Chitosan has good bioadhesive properties and biodegradability (Jiang Tingda. Chitosan. Beijing: Chemical Industry Press 2001: 1~32), which can enhance penetration and enzyme inhibition, so it was developed into The delivery carrier of biomacromolecular drugs to increase the effective concentration of drugs in the body and improve the curative effect. Compared with other types of polymer drug carriers, chitosan is not only rich in raw materials, but also has the advantages of low cost, biodegradability, good biocompatibility and bioaffinity, non-toxic, and easy chemical modification. It also has th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/36A61K47/02A61K9/14A61K31/513A61P35/00
Inventor 沈鹤柏马经纬陈艳
Owner SHANGHAI NORMAL UNIVERSITY
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