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Homotype semicystinol diagnositic kit and homotype semicystinol concentration investigating method

A technology of homocysteine ​​and a diagnostic kit, which is applied in the field of medical testing and determination, can solve the problems of complicated operation, long test time, large variation of test data, etc.

Inactive Publication Date: 2007-12-19
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its characteristics are similar to those of high-performance liquid chromatography, but it has the disadvantages of complicated operation, long test time and large variation of test data.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] The homocysteine ​​diagnosis / measurement reagent of this embodiment is a single reagent, including:

[0052] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0053] Stabilizer 50mmol / L

[0054] Reduced coenzyme 0.25mmol / L

[0055] Cystathionine β synthase 2000U / L

[0056] γ-Cystathionase 5000U / L

[0057] Cysteine ​​desulfhydrylase 5000U / L

[0058] Hydroxybutyrate dehydrogenase 30000U / L

[0059] Glutamate dehydrogenase 50000U / L

[0060] Lactate dehydrogenase 10000U / L

[0061] Serine 50mmol / L

[0062] α-ketoglutarate 16mmol / L

[0063] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0064] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the tested homocysteine ​​s...

Embodiment 2

[0067] The homocysteine ​​diagnostic reagent of this embodiment is a dual reagent, including:

[0068] Reagent 1

[0069] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0070] Stabilizer 50mmol / L

[0071] Reduced coenzyme 0.25mmol / L

[0072] Hydroxybutyrate dehydrogenase 40000U / L

[0073] Glutamate dehydrogenase 40000U / L

[0074] Lactate dehydrogenase 15000U / L

[0075] α-ketoglutarate 12mmol / L

[0076] Reagent 2

[0077] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0078] Stabilizer 50mmol / L

[0079] Cystathionine β synthase 3000U / L

[0080] γ-Cystathionase 6000U / L

[0081] Cysteine ​​desulfhydrylase 6000U / L

[0082] Serine 20mmol / L

[0083] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0084] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelen...

Embodiment 3

[0087] The homocysteine ​​diagnostic reagent of this embodiment is three reagents, including:

[0088] Reagent 1

[0089] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0090] Stabilizer 50mmol / L

[0091] Reduced coenzyme 0.25mmol / L

[0092] α-ketoglutarate 10mmol / L

[0093] Reagent 2

[0094] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0095] Stabilizer 50mmol / L

[0096] Hydroxybutyrate dehydrogenase 60000U / L

[0097] Glutamate dehydrogenase 60000U / L

[0098] Lactate dehydrogenase 20000U / L

[0099] Reagent 3

[0100] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0101] Stabilizer 50mmol / L

[0102] Cystathionine β synthase 4000U / L

[0103] γ-Cystathionase 4000U / L

[0104] Cysteine ​​desulfhydrylase 4000U / L

[0105] Serine 20mmol / L

[0106] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0107] When m...

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Abstract

A diagnosis kit of homocysteine consists of compositions as buffer solution, serine, alpha-ketoglutaric acid, cystathionine beta-synthase, upsilon-cystathionine enzyme, homocysteine desulfydrase, hydroxybutyric dehydrogenase, glutamate dehydrogenerase, lactic dehydrogenase, reduced coenzyme and stabilizer. Its method for determining concentration of homecysteine is also disclosed.

Description

technical field [0001] The invention relates to a homocysteine ​​diagnostic kit, and at the same time, the invention also relates to a method for determining the concentration of homocysteine, which belongs to the technical field of medical examination and determination. Background technique [0002] There are many methods for measuring homocysteine ​​in plasma, including high performance liquid chromatography (HPLC), amino acid analyzer assay, ion chromatography, enzyme-linked immunoassay (EIA), capillary gas chromatography-mass spectrometry, fluorescence Polarization immunoassay (FPIA), electrochemical method and enzymatic method, etc. [0003] 1. High-performance liquid chromatography (HPLC): It is a classic reference method, but it has the disadvantages of complicated operation, long test time, and large variation of test data. In clinical application, it is gradually adopted by enzyme-linked immunoassay, Fluorescence polarization analysis and enzymatic methods supersed...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/78G01N33/50G01N33/52G01N33/68C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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