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Application of embedded lactobacillus in animal feed and bacteriostatic promoting production

A technology of animal feed and lactic acid bacteria, applied in animal feed, animal feed, application, etc., can solve the problems of difficult preparation and long-term storage, high difficulty in cultivation and proliferation, poor external resistance, etc., and achieve strong anti-microbial decomposition performance and chemical stability Good performance and extended service life

Inactive Publication Date: 2008-01-09
HENAN AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

A large number of studies have confirmed that the probiotics produced by microorganisms such as lactic acid bacteria play a significant role in maintaining the balance of animal digestive tract microflora, inhibiting the growth of harmful bacteria, reducing the occurrence of diarrhea and other digestive tract diseases, and promoting production. However, lactic acid bacteria are anaerobic Bacteria, which are difficult to cultivate and proliferate, have poor resistance to the outside world, are difficult to prepare and store for a long time, and reduce their role in animals

Method used

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  • Application of embedded lactobacillus in animal feed and bacteriostatic promoting production

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Embodiment 1, the preparation of embedding lactobacillus:

[0012] Sodium alginate and polyvinyl alcohol were selected as embedding agents respectively, and the specific steps were as follows:

[0013] (1) Culture of lactic acid bacteria

[0014] Inoculate 0.1ml of lactic acid bacteria (Lactobacillus casei) into 5ml of deoxygenated MRS medium, and culture it in a 37°C incubator for 18h. After the cultivation, the obtained bacterial concentration was 1.072×10 8 pieces / ml.

[0015] MRS anaerobic medium: tryptone 10g / l, beef extract 10g / l, yeast extract 5g / l, glucose 20g / l, Tween (80) 1ml / l, K 2 HPO2g / l, sodium acetate 5g / l, triammonium citrate 2g / l, MgSO 4 0.2g / l, MnSO 4 0.05g / l, autoclave at 120°C for 20min.

[0016] (2) Preparation of sodium alginate immobilized pellets

[0017] 1. Embedding material

[0018] Sodium Alginate, 1% CaCl 2 , sterile water, 50% sucrose solution

[0019] 2. Experimental method

[0020] First, use an electronic balance to accurately...

Embodiment 2

[0049] Embodiment 2, the feeding test selects 24 dwarf Gushi laying hens at the later stage of the egg production peak, and randomly divides them into 3 groups, with 8 chickens in each group. The control group was fed with common laying hen feed (balanced with sterile culture solution and diluent), the test I group added fresh samples of lactic acid bacteria (balanced with diluent) in the diet of the control group, and the experimental group II was fed with the diet of the control group. Add 0.1% embedding lactic acid bacteria (balanced with aseptic culture solution) in the medium, and the test period is 34 days. The results showed that the diarrhea rate of chickens in the two test groups decreased by 8.5-23.4% compared with the control group, and the egg production rate and egg weight increased by 2.0-6.7% and 4.7-7.8% respectively compared with the control group. The results are shown in Table 1.

[0050] At the end of the feeding experiment, all the participating chickens w...

Embodiment 3

[0059] Embodiment 3, the feeding test selects 24 dwarf Gushi laying hens in the later stage of the egg production peak, and adds 0.01% embedded lactic acid bacteria in the diet of the control group except that the test II group, its grouping situation and experimental conditions are the same as in Example 2 .

[0060] The results showed that the diarrhea rate of chickens in the two test groups decreased by 8-21% compared with the control group, and the egg production rate and egg weight increased by 3-6% and 4-7% respectively compared with the control group. At the end of the feeding experiment, all the participating chickens were subjected to the Shigella Escherichia coli challenge protection experiment. The mortality rate of the chickens in the control group was 31%, while none of the chickens in the two test groups died. During the challenge experiment, the egg production rate and egg weight of chickens added with embedded lactic acid bacteria group increased by 35% and 11%...

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Abstract

The present invention belongs to the field of microbial application technology, relates to an application of embedded lactic acid bacteria in animal feed. It is characterized by that in animal feed a kind of embedded lactic acid bacteria can be added, the added quantity of embedded lactic acid bacteria is 0.01-0.5% of animal feed weight. Said invention can be used as additive of animal feed, so that it has extensive application.

Description

technical field [0001] The invention belongs to the technical field of microorganism application and relates to the application of embedding lactic acid bacteria. Background technique [0002] At present, farmers and feed producers often add antibiotics to animal feed as antibacterial and production-promoting agents. Although the production performance of animals has been improved, antibiotics have the disadvantages of residues, drug resistance, drug resistance, and harm to human health. , so it is imperative to develop new and safe alternatives to antibiotics. Beneficial microorganisms such as lactic acid bacteria are a large group of bacteria that have been used by humans as food microorganisms since ancient times, and are widely used in fermented dairy products, fruits, vegetables, bread made from rye flour, and animal feed. A large number of studies have confirmed that the probiotics produced by microorganisms such as lactic acid bacteria play a significant role in main...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16A23K10/18
Inventor 尹清强郑秋红路玲冯华左瑞雨梁萧静
Owner HENAN AGRICULTURAL UNIVERSITY
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