Extracting and purifying technology for haw flavonoids substances

Abstract

Disclosed is an extraction and purification process of hawthorn flavonoid ingredients. The process is practiced in steps that firstly, hawthorn decoction pieces are dried and processed into particles with 3-8 mm diameter; the particles with ethanol with 70 percent of concentration are refluxed and extracted for three times; for the first time the particles and the ethanol with eight times of weight of the particles are extracted for two hours, and for the second and the third times, the particles and the ethanol with 6 times of weight of the particles are extracted for one hour; the liquid extractive is combined and decompressed for ethanol recovering, and then are condensed to extractive concentrated solution; secondly, water is added into the extractive concentrated solution until every milliliter of the extractive concentrated solution contains 0.1g of crude drug, the pH is regulated between 3 and 4, and then filtrate is produced after the solution is filtered; thirdly, the filtrate is made to pass through a D-101 macroporous resin column for adsorption, water washing and ethanol elution; lastly, the ethanol eluant is collected and condensed to the eluant with 1.1g per milliliter in consistency, and then the hawthorn extractive is produced after the condensed eluant is dried by spray. With the hawthorn extraction method, the total flavone transfer rate of hawthorn can be achieved to 79.5 per cent, and the total flavone content in the hawthorn extractive is calculated by rutin to be 24.6 per cent.

Description

technical field

[0001] The invention relates to an extraction method of traditional Chinese medicine, in particular to an extraction method of hawthorn and its extract. Background technique

[0002] Hawthorn is the dried and mature fruit of Crataegus pinnatifida Bge.var major.N.E.Br. or C. pinnatifida Bge. of the genus Hawthorn in the family Rosaceae, which was recorded in the 2005 edition of "Chinese Pharmacopoeia". It has the effects of eliminating food accumulation, promoting blood circulation and removing blood stasis. More than 150 kinds of components have been found and isolated from hawthorn (pulp, core and leaves), the main components are flavonoids, organic acids and so on. Flavonoids are the main active ingredients of hawthorn, and hyperoside, rutin, quercetin, and vitexin have been isolated. In recent years, raw hawthorn has been used in the clinical treatment of diseases such as hypertension, coronary heart disease and hyperlipidemia. Modern pharmacological st...

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