Agrobacterium mediated onion epidermis cell conversion method

A technology mediated by Agrobacterium and epidermal cells, applied in the direction of introducing foreign genetic material using vectors, recombinant DNA technology, etc., can solve the problems of high cost, high equipment requirements, and difficulty in onion epidermal cells, and achieve high-efficiency transformation and low cost. cost effect
CN101113458AInactive Publication Date: 2008-01-30CROP RES INST GUANGDONG ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CROP RES INST GUANGDONG ACAD OF AGRI SCI
Publication Date
2008-01-30
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses an onion epidermal cell transformation method mediated by agrobacterium. Onion monolayer endocuticula is dipped and floated in an infiltration medium containing with agrobacterium and is fully contacted with agrobacterium for 5-30min for transformation and then is picked up and laid in an MS solid culture medium for culture for 16-24 hours under a temperature of 25 DEG C, 16-hour lighting and 8-hour darkness. The invention needs no expensive instrument such as a gene gun, etc, no expensive experimental carrier materials such as gold powder, etc. but a super clean working platform and relative agrobacterium strains can realize high effective transformation of onion epidermal cell in the simplest experimental environment. The whole course only requires four days with low cost and the transformation rate thereof can reach 80 percent.
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Description

technical field

[0001] The invention relates to a method for genetic transformation of onion epidermal cells, in particular to a simple and efficient method for transforming exogenous genes into epidermal cells in onion bulb leaves mediated by Agrobacterium tumefaciens. Background technique

[0002] The inner epidermis of onion bulb leaves has the advantages of convenient material collection, obvious cell structure, transparency, no chloroplast interference, easy observation, and in vivo detection. It is used as a plant genetic transformation receptor and is widely used in protein subcellular localization and promoter activity. In recent years, with the rapid development of cell biology and molecular biology, it has become increasingly important to use onion epidermal cells as receptors to study the functions and characteristics of genes, proteins, promoters, etc. A hotspot in molecular biology research.

[0003] At present, the genetic transformation of onion epidermal cel...

Claims

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