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Method for preparing high-purity liquorice chalcone A

A licorice chalcone, high-purity technology, applied in the field of medical drugs and chemical industry, can solve the problems of high cost and complicated operation, and achieve the effect of low cost and simple process

Inactive Publication Date: 2008-02-06
HUAZHONG UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The purpose is to overcome the defects of complicated operation and high cost in the existing method, so as to be suitable for low-cost industrial production

Method used

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  • Method for preparing high-purity liquorice chalcone A
  • Method for preparing high-purity liquorice chalcone A

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Weigh 1Kg of dried and pulverized licorice powder, soak it with 20 times the amount of 80% ethanol at room temperature for 2 hours, then extract by cold infiltration method, the percolation speed is 10mL / min, the percolation liquid is concentrated under reduced pressure, and concentrated to a specific gravity of 1.08 (20°C), stir and extract 3 times with water-saturated n-butanol at a ratio of 1:1, combine the extracts and concentrate under reduced pressure to recover n-butanol, the obtained concentrate is adsorbed with 500 g of macroporous D101 resin, and eluted with a gradient of ethanol and water. , wherein the proportion of water in ethanol water is from 100wt%, the interval is 20wt%, the gradient decreases to 0wt%, the effluent is checked by thin-layer chromatography detection method, the effluent containing licorice A is collected, and the licorice is concentrated under reduced pressure. The concentrate of licorone A was crystallized with isopropyl ether-acetone to...

Embodiment 2

[0024] Weigh 1Kg of dried and pulverized licorice powder, extract 2 times by mass ratio of 1:3 and 1:2 with absolute ethanol, each time for 1 hour, filter, merge the filtrate, recover ethanol under reduced pressure, add water to the specific gravity after concentration 1.10 (20°C), stir and extract 3 times with ethyl acetate at a ratio of 1:1, combine the extracts, recover ethyl acetate under reduced pressure, the obtained concentrate is adsorbed with 500 g of polyamide, and eluted with a gradient of ethanol and water, wherein water accounts for ethanol. The proportion of water is from 100wt%, the interval is 15wt%, and the gradient decreases to 0wt%. The effluent is checked by thin-layer chromatography detection method, and the effluent containing licochalone A is collected, and concentrated under reduced pressure to obtain licochalone A concentrate , and then crystallized from ethanol-isopropyl ether to obtain 2.07 g of pure licorice chalcone A with a purity of 91.82%.

Embodiment 3

[0026] Weigh 1Kg of dried and pulverized licorice powder, extract 3 times with 10 times, 5 times, and 5 times of 80% acetone water respectively. The first extraction is 3 hours, the second extraction is 1 hour, and the third extraction is 1 hour. The extract was recovered under reduced pressure at 40°C with acetone, concentrated to a specific gravity of 1.05 (20°C), continuously extracted with ethyl acetate at a ratio of 1:3, and the extract was concentrated. Elution, wherein the proportion of water in ethanol water is from 100wt%, the interval is 20wt%, the gradient is decreased to 0wt%, the effluent is checked by thin-layer chromatography detection method, the effluent containing licochalone A is collected, and concentrated under reduced pressure to obtain The licorice chalcone A concentrate was crystallized through isopropyl ether-acetone to obtain 2.73 g of licorice chalcone A pure product with a purity of 91.77%.

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Abstract

The invention relates to a preparation process for high pure licochalcone A. The technique procedures of the invention are as follows : at first, the liquorice is pulverized and then extracted 1 to 3 times with the solvent using cold percolation, warm soaking or refluence extraction process; the extraction liquid is emerged and made a pressure reduction concentration; then the extraction liquid is extracted again by the organic solvent and the extracted liquid is made a pressure reduction concentration, and the organic solvent is reclaimed, the concentrate adopts the column chromatography separation and is cleaned by water, alcohol, methanol, acetone or the mixing liquid thereof, the effluent of licochalcone A is collected to be concentrated and crystallized by the organic solvent to obtain the high pure licochalcone A. The invention has simple process, less procedures, the solvent, the column stuffing as well as the eluent which all can be repeatedly used, and is a preparation process with low cost, high purity, easy magnification and recycle type; the acquired licochalcone A is orange needle crystals with no lower than 90 percent of purity.

Description

technical field [0001] The invention belongs to the fields of medical medicine and chemical industry, and particularly relates to a method for obtaining high-purity (>90%) Licochalcone A by extracting, separating and purifying from Glycyrrhiza. Background technique [0002] Licochalcone A (Licochalcone A) is a kind of chalcone in licorice, and its structure is: [0003] [0004] In recent years, with the continuous in-depth research on licorice chalcone A, it has been found that in addition to its antioxidant and free radical scavenging activities, it also has anti-leishmaniasis (especially kala-azar), anti-inflammatory, anti-HIV, Anti-tumor, antibacterial, immune promotion and other functions have a wide range of uses in the food and pharmaceutical industries. Literature Biochemical and Biophysical Research Communications 2004322263-270 reported that licorice A can inhibit the proliferation of hormone-independent PC-3 prostate cancer cells, suggesting that licorice A...

Claims

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Application Information

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IPC IPC(8): C07C49/835C07C45/78A61K36/484
Inventor 余龙江崔永明敖明章叶玉平梁晓荣
Owner HUAZHONG UNIV OF SCI & TECH
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